Taq & High-Fidelity DNA Polymerase Comparison Chart

Specifications and pricing of commercially available Taq DNA Polymerases and High-Fidelity DNA Polymerases in Canada.

Max price per 50 ul reaction is based on the list price (CAD) of the smallest pack size for each product and each supplier available in Canada. Cost/reaction does not include the cost for dNTPs, other reagent or plastic consumables.

Last updated on 2018-09-15.

Product Name (suppller)	Cat. No	HotStart	Category	Fidelity	Extension Rate	Max Amplicon size (gDNA/plasmid)	Max Price (CAD) per 50 ul rx
EasyTaq® DNA Polymerase	AP111	No	Standard Taq	1x	1-2 kb/min	4 kb	0.25 $
Taq DNA Polymerase (NEB)	M0267	No	Standard Taq	1x	1 kb/min	6 kb	0.27 $
TransFast® Taq DNA Polymerase (6 kb/min)	AP101	No	Standard Taq	1x	6 kb/min	4 kb	0.24 $
TransStart® Taq DNA Polymerase	AP141	Yes	Standard Taq	18x	1-2 kb/min	15 kb	0.43 $
TransTaq®-T DNA Polymerase	AP122	Yes	Enzyme Blends	18x	1-2 kb/min	8 kb	0.50 $
EasyPfu DNA Polymerase	AP211	No	High-Fidelity	18x	0,5 kb/min	6 kb / 10 kb	0.40 $
TransStart® TopTaq DNA Polymerase	AP151	Yes	Standard Taq	18x	1-2 kb/min	15 kb	0.49 $
TransTaq® (HiFi) DNA Polymerase High-Fidelity	AP131	Yes	Enzyme Blends	18x	1-2 kb/min	15 kb	0.68 $
Taq DNA Polymerase (Qiagen)	201203	No	Standard Taq	1x	2–4 kb/min	N/A	0.79 $
Taq DNA Polymerase, recombinant (Invitrogen)	10342053	No	Standard Taq	1x	1 kb/min	5 kb	0.80 $
HotStarTaq DNA Polymerase (Qiagen)	203203	Yes	Standard Taq	1x	2–4 kb/min	N/A	0.93 $
TransStart® KD Plus (Ultra-HiFi) DNA Polymerase	AP301	Yes	Ultra-High Fidelity	108x	1 kb/min	15 kb / 20 kb	1.07 $
Q5® High-Fidelity DNA Polymerase (NEB)	M0491	No (available)	Ultra-High Fidelity	100x	2-6 kb/min	10 kb / 20 kb	1.33 $
Q5® Hot Start High-Fidelity DNA Polymerase (NEB)	M0493	Yes	Ultra-High Fidelity	100x	2-6 kb/min	10 kb / 20 kb	1.66 $
TransStart® FastPfu (High-Fidelity) DNA Polymerase	AP221	Yes	High-Fidelity	54x	2-4 kb/min	15 kb / 20 kb	1.19 $
KOD DNA Polyerase (EMD/Toyobo)	71085-3	No (available)	High-Fidelity	3.7x	4-6 kb/min	2 kb / 6 kb	1.50 $
Phusion HotStart II High-Fidelity DNA Polymerase (ThermoFisher)	F549	Yes	High-Fidelity	52x	2-4 kb/min	10 kb / 20 kb	1.68 $
Platinum™ SuperFi™ DNA Polymerase (ThermoFisher)	12351010	Yes	Ultra-High Fidelity	100x	2-6 kb/min	10 kb / 20 kb	2.62 $
TransStart® FastPfu FLY (Ultra-HiFi) DNA Polymerase	AP231	Yes	Ultra-High Fidelity	108x	2-6 kb/min	15 kb / 20 kb	1.44 $
Platinum® Taq DNA Polymerase HiFi (Life)	11304029	Yes	Enzyme Blends	6x	1 kb/min	15 kb / 20 kb	2.75 $
Accuprime Pfx (KOD)(Invitrogen)	12344024	Yes	High-Fidelity	26x	1 kb/min	12 kb	2.94 $
PfuUltra II Fusion HS (Agilent)	600670	Yes	High-Fidelity	20x	2-4 kb/min	19 kb	3.40 $