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TransScript All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (+gDNA Removal) – AT341

(2 customer reviews)

364.00$659.00$ CAD

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All-in-One First-Strand cDNA Synthesis SuperMix for qPCR, with One-Step gDNA Remover

TransScript All-in-One First-Strand cDNA Synthesis SuperMix for qPCR provides all the necessary components for cDNA synthesis from total RNA or mRNA. It is provided at 5x concentration and used at 1x concentration by adding gDNA remover, RNA and H2O. Simultaneous genomic DNA removal and cDNA synthesis are performed in one tube. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85°C for 5 seconds. The resulting cDNA are suitable for qPCR and not for regular PCR.


Advantages of using TransScript All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal)

  • Simultaneous genomic DNA removal and cDNA synthesis in one tube.
  • The optimal ratio of oligo(dT)18 primer to random primer(N9) for qPCR ready cDNA.
  • qPCR ready cDNA in 15 minutes.
  • cDNA up to 250 bp
  • Reactions are carried at 42°C

 

TGAT341 content


Manual and Datasheet

AT341 TransScript® All-in-One First-Strand cDNA Synthesis SuperMix for qPCR(One-Step gDNA Removal)


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Additional information

Format

50 x 20 ul rx, 100 x 20 ul rx

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Reviews for TransScript All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (+gDNA Removal) – AT341

2 reviews for TransScript All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (+gDNA Removal) - AT341

  1. srcivicbio

    User review from CHU Ste-Justine, Montreal, QC submitted by email:

    Salut Simon,
    je suis prête à essayer ton kit de T7 pour voir s’il fonctionne bien. Je vais aussi acheter ta Transcript All-in-one first strand cDNA… (TGAT341-01) je l’ai essayée et elle marche bien (dans mon cas mieux que la RevertAid de fisher).

  2. virginie.sanguin (verified owner)

    User review from Goodman Cancer Center, Mcgill University, Montréal, december 06, 2017.
    The all in one kit was as effective as other cDNA synthesis kits we have used in the past with the additional benefit of all components in a ready to use master mix, and requires a thermocycler program with a far shorter duration.

    • Simon Roy (verified owner)

      Merci Virignie! J’espère que vos résultats de qPCR sont aussi bons que la RTase! J’ai mis des échantillons de TIP qPCR AQ141 dans votre dernier envoi afin que votre laboratoire puisse l’essayer sans frais 😉

      Voici maintenant ta récompense cashback x2, utilisable lors de ta prochaine commande!: virginie AT341

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