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TransScript All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (+gDNA Removal) – AT341

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TS All-in-One First-Strand cDNA Synthesis SuperMix for qPCR, with One-Step gDNA Remover

TransScript All-in-One First-Strand cDNA Synthesis SuperMix for qPCR provides all the necessary components for cDNA synthesis from total RNA or mRNA. It is provided at 5x concentration and used at 1x concentration by adding gDNA remover, RNA and H2O. Simultaneous genomic DNA removal and cDNA synthesis are performed in one tube. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85°C for 5 seconds. The resulting cDNA are suitable for qPCR and not for regular PCR.


Advantages of using TransScript All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal)

  • Simultaneous genomic DNA removal and cDNA synthesis in one tube.
  • The optimal ratio of oligo(dT)18 primer to random primer(N9) for qPCR ready cDNA.
  • qPCR ready cDNA in 15 minutes.
  • cDNA up to 250 bp
  • Reactions are carried at 42°C

 

TGAT341 content


Manual and Datasheet

AT341 TransScript® All-in-One First-Strand cDNA Synthesis SuperMix for qPCR(One-Step gDNA Removal)


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Additional information

Format

50 x 20 ul rx, 100 x 20 ul rx

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