Description
DNA Cloning Service for Expression Vectors
This cloning service provided by Civic Bioscience is for cloning any sequence into an expression vector of your choice (provided by yourself) or one of TransGen Biotech‘s pEASY Expression Vectors.
Pricing Cost of CivicBio's DNA Cloning Services
DNA %GC / kb | < 1 kb | 1-2 kb | 2-3 kb | 3-4 kb | 4-5 kb | > 5 kb |
---|---|---|---|---|---|---|
20-30 % GC | 320 $ | 360 $ | 400 $ | 440 $ | 610 $ | 610 $* + 400$/extra kb |
31-40 % GC | 280 $ | 320 $ | 360 $ | 400 $ | 550 $ | 550 $* + 250$/extra kb |
41-60 % GC | 250 $ | 290 $ | 330 $ | 370 $ | 500 $ | 500 $* + 150$/extra kb |
61-70 % GC | 280 $ | 320 $ | 360 $ | 400 $ | 550 $ | 550 $* + 200$/extra kb |
71-80 % GC | 310 $ | 350 $ | 410 $ | 460 $ | 610 $ | 610 $* + 350$/extra kb |
81-85 % GC | 350 $ | 400 $ | 450 $ | 530 $ | 750 $ | 750 $* + 500$/extra kb |
86-90 % GC | 400 $* | 460 $* | 560 $* | 650 $* | 900 $* | 900 $* + 750$/extra kb |
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Rare DNA clones available from CivicBio | 2019-05-16
Cat. No. | Species | GOI | Isoform | Gene ID | Mutation | N-ter tag | C-ter tag | Vector 1 | Antibiotic R | Note(s) |
---|---|---|---|---|---|---|---|---|---|---|
CB-DNA-0001 | human | RAD51 | Q313K | 3xFlag | none | pCMV | Kananmycin | |||
CB-DNA-0002 | human | POLA2 | EGFP | none | pcDNA3/DOriSV40 | Ampicillin | ||||
CB-DNA-0003 | human | PRIM2 | EGFP | none | pcDNA3/DOriSV40 | Ampicillin | ||||
CB-DNA-0004 | human | CXorf57 | X1 | EGFP | none | pcDNA3/DOriSV40 | Ampicillin | |||
CB-DNA-0004 | human | CXorf57 | X2 | EGFP | none | pcDNA3/DOriSV40 | Ampicillin | |||
CB-DNA-0005 | human | CXorf57 | X1 | none | none | pLVX | Ampicillin | |||
CB-DNA-0006 | human | RAD51 | EGFP | none | pcDNA3/DOriSV40 | Ampicillin | ||||
CB-DNA-0007 | human | ICE2 | EGFP | none | pcDNA3/DOriSV40 | Ampicillin | ||||
CB-DNA-0008 | human | BLM | K695A | EGFP | none | pCMV/DOriSV40 | Kananmycin | |||
CB-DNA-0009 | human | BLM | EGFP | none | pCMV/DOriSV40 | Kananmycin | ||||
CB-DNA-0010 | human | BLM | K695A | EGFP | none | pCMV | Kananmycin | |||
CB-DNA-0011 | human | BLM | EGFP | none | pCMV | Kananmycin | ||||
CB-DNA-0012 | human | POLA1 | EGFP | none | pcDNA3/DOriSV40 | Ampicillin | ||||
CB-DNA-0013 | human | RPA1 | EGFP | none | pcDNA3/DOriSV40 | Ampicillin | ||||
CB-DNA-0014 | human | RPA2 | EGFP | none | pcDNA3/DOriSV40 | Ampicillin | ||||
CB-DNA-0015 | human | RPA3 | EGFP | none | pcDNA3/DOriSV40 | Ampicillin | ||||
CB-DNA-0016 | human | PRIM1 | EGFP | none | pcDNA3/DOriSV40 | Ampicillin | ||||
CB-DNA-0017 | human | POLA1 | BirA-Flag | none | pcDNA5/FRT/DEST | Ampicillin | Stabilized DNA sequence --> doesn't recombine. Ultra-low DNA yield. | |||
CB-DNA-0018 | human | RPA1 | BirA-Flag | none | pcDNA5/FRT/DEST | Ampicillin | ||||
CB-DNA-0019 | human | RPA2 | BirA-Flag | none | pcDNA5/FRT/DEST | Ampicillin | ||||
CB-DNA-0020 | human | RPA3 | BirA-Flag | none | pcDNA5/FRT/DEST | Ampicillin | ||||
CB-DNA-0021 | human | PRIM1 | BirA-Flag | none | pcDNA5/FRT/DEST | Ampicillin | ||||
CB-DNA-0022 | human | SLFN11 | EGFP | none | pcDNA3/DOriSV40 | Ampicillin | ||||
CB-DNA-0023 | human | SLFN11 | K605M+D668A | EGFP | none | pcDNA3/DOriSV40 | Ampicillin | |||
CB-DNA-0024 | human | SLFN11 | none | none | pLVX | Ampicillin | ||||
CB-DNA-0025 | human | BRCA4 | NLS | Flag | pcDNA3/DOriSV40 | Ampicillin | ||||
CB-DNA-0026 | human | SMARCAL1 | EGFP | none | pcDNA3/DOriSV40 | Ampicillin | ||||
CB-DNA-0027 | human | SMARCAL1 | R764Q | EGFP | none | pcDNA3/DOriSV40 | Ampicillin | |||
CB-DNA-0028 | human | ZRANB3 | X2 variant | EGFP | none | pcDNA3/DOriSV40 | Ampicillin | |||
CB-DNA-0029 | human | ZRANB3 | isoform 3 | EGFP | none | pcDNA3/DOriSV40 | Ampicillin | |||
CB-DNA-0030 | human | ZRANB3 | isoform 1 | EGFP | none | pcDNA3/DOriSV40 | Ampicillin | |||
CB-DNA-0031 | human | ZRANB3 | isoform 1 | K65R | EGFP | none | pcDNA3/DOriSV40 | Ampicillin | ||
CB-DNA-0032 | human | WRNIP1 | isoform 1 | EGFP | none | pcDNA3/DOriSV40 | Ampicillin | |||
CB-DNA-0033 | human | WRNIP1 | x2 variant | EGFP | none | pcDNA3/DOriSV40 | Ampicillin | |||
CB-DNA-0034 | human | WRNIP1 | isoform 1 | T294A | EGFP | none | pcDNA3/DOriSV40 | Ampicillin | ||
CB-DNA-0035 | human | HLTF | isoform 1 | D557A+E558A | EGFP | none | pcDNA3/DOriSV40 | Ampicillin | ||
CB-DNA-0036 | human | NRF2 | none | none | pcDNA3 | Ampicillin | ||||
CB-DNA-0037 | human | CHIP | EGFP | none | pcDNA3/DOriSV40 | Ampicillin | ||||
CB-DNA-0038 | human | CHIP | K30A | EGFP | none | pcDNA3/DOriSV40 | Ampicillin | |||
CB-DNA-0039 | human | MC2R | Myc | none | pcDNA5/FRT | Ampicillin | available in a bicistronic vector with MRAPalpha or beta| T143 and N-glycosylation mutants are available | |||
CB-DNA-0040 | human | MRAP1 | MRAPa | Flag | pcDNA5/FRT | Ampicillin | many tagged versions available | |||
CB-DNA-0041 | human | MRAP1 | MRAPb | Flag | pcDNA5/FRT | Ampicillin | many tagged versions available | |||
CB-DNA-0056 | human | MRAP2 | Flag | pcDNA5/FRT | Ampicillin | |||||
CB-DNA-0042 | human | CHD2 | long isoform | HA | none | pCS2 | Ampicillin | Stabilized DNA sequence --> doesn't recombine. | ||
CB-DNA-0043 | human | TWIST1 | none | pEASY-Blunt3 | Ampicillin | 2,1 kb genomic region containing exon 1 and 2 | ||||
CB-DNA-0044 | human | PTPRZ1 | 5803 | mCherry | pCDH-EF1a-RedLuc | pGK | Ampicillin | ||||
CB-DNA-0045 | human | PTPRZ1 | 5803 | mOrange2 | pCDH-EF1a-RedLuc | pGK | Ampicillin | ||||
CB-DNA-0046 | human | CDK6 | 1021 | mTurquoise2 | pCDH-EF1a-RedLuc | pGK | Ampicillin | ||||
CB-DNA-0047 | human | DBF4B | isoform 2 | 80174 | mScarlet-I | pCDH-EF1a-RedLuc | pGK | Ampicillin | |||
CB-DNA-0048 | human | DBF4B | isoform 1 | 80174 | pCDH-EF1a-RedLuc | pGK | Ampicillin | ||||
CB-DNA-0049 | human | EZH2 | 2146 | CreiLOV | pCDH-EF1a-RedLuc | pGK | Ampicillin | ||||
CB-DNA-0050 | human | MCM7 | isoform 1 | 4176 | mKate2 | pCDH-EF1a-RedLuc | pGK | Ampicillin | |||
CB-DNA-0051 | human | VGF1 | 7425 | mBlueberry2 | pCDH-EF1a-RedLuc | pGK | Ampicillin | ||||
CB-DNA-0052 | human | XRCC2 | 7516 | mGarnett2 | pCDH-EF1a-RedLuc | pGK | Ampicillin | ||||
CB-DNA-0053 | human | AKT1 | T308A-S473A | turboGFP | Ampicillin | |||||
CB-DNA-0054 | human | AKT1 | T308D-S473D | turboGFP | Ampicillin | |||||
CB-DNA-0055 | human | AKT1 | turboGFP | Ampicillin |
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