DNA Cloning Service in Expression Vector – CB-CLONE

Description

DNA Cloning Service for Expression Vectors

This cloning service provided by Civic Bioscience is for cloning any sequence into an expression vector of your choice (provided by yourself) or one of TransGen Biotech‘s pEASY Expression Vectors.

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Pricing Cost of CivicBio's DNA Cloning Services

DNA %GC / kb	< 1 kb	1-2 kb	2-3 kb	3-4 kb	4-5 kb	> 5 kb
20-30 % GC	320 $	360 $	400 $	440 $	610 $	610 $* + 400$/extra kb
31-40 % GC	280 $	320 $	360 $	400 $	550 $	550 $* + 250$/extra kb
41-60 % GC	250 $	290 $	330 $	370 $	500 $	500 $* + 150$/extra kb
61-70 % GC	280 $	320 $	360 $	400 $	550 $	550 $* + 200$/extra kb
71-80 % GC	310 $	350 $	410 $	460 $	610 $	610 $* + 350$/extra kb
81-85 % GC	350 $	400 $	450 $	530 $	750 $	750 $* + 500$/extra kb
86-90 % GC	400 $*	460 $*	560 $*	650 $*	900 $*	900 $* + 750$/extra kb

Estimative pricing of DNA cloning service costs depending on GC percentage and DNA lenght. Actual pricing may vary depending on factors such as the presence of repetitive sequences, the destination vector, toxicity and required competent cells. A 30$ extra charge may apply for the use of specialized competent cells. An extra charge is also applied in the event that the cloned DNA consists of a gene fusion (50$/internal junction).

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Request your Service Quote

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Rare DNA clones available from CivicBio | 2019-05-16

Cat. No.	Species	GOI	Isoform	Gene ID	Mutation	N-ter tag	C-ter tag	Vector 1	Antibiotic R	Note(s)
CB-DNA-0001	human	RAD51			Q313K	3xFlag	none	pCMV	Kananmycin
CB-DNA-0002	human	POLA2				EGFP	none	pcDNA3/DOriSV40	Ampicillin
CB-DNA-0003	human	PRIM2				EGFP	none	pcDNA3/DOriSV40	Ampicillin
CB-DNA-0004	human	CXorf57	X1			EGFP	none	pcDNA3/DOriSV40	Ampicillin
CB-DNA-0004	human	CXorf57	X2			EGFP	none	pcDNA3/DOriSV40	Ampicillin
CB-DNA-0005	human	CXorf57	X1			none	none	pLVX	Ampicillin
CB-DNA-0006	human	RAD51				EGFP	none	pcDNA3/DOriSV40	Ampicillin
CB-DNA-0007	human	ICE2				EGFP	none	pcDNA3/DOriSV40	Ampicillin
CB-DNA-0008	human	BLM			K695A	EGFP	none	pCMV/DOriSV40	Kananmycin
CB-DNA-0009	human	BLM				EGFP	none	pCMV/DOriSV40	Kananmycin
CB-DNA-0010	human	BLM			K695A	EGFP	none	pCMV	Kananmycin
CB-DNA-0011	human	BLM				EGFP	none	pCMV	Kananmycin
CB-DNA-0012	human	POLA1				EGFP	none	pcDNA3/DOriSV40	Ampicillin
CB-DNA-0013	human	RPA1				EGFP	none	pcDNA3/DOriSV40	Ampicillin
CB-DNA-0014	human	RPA2				EGFP	none	pcDNA3/DOriSV40	Ampicillin
CB-DNA-0015	human	RPA3				EGFP	none	pcDNA3/DOriSV40	Ampicillin
CB-DNA-0016	human	PRIM1				EGFP	none	pcDNA3/DOriSV40	Ampicillin
CB-DNA-0017	human	POLA1				BirA-Flag	none	pcDNA5/FRT/DEST	Ampicillin	Stabilized DNA sequence --> doesn't recombine. Ultra-low DNA yield.
CB-DNA-0018	human	RPA1				BirA-Flag	none	pcDNA5/FRT/DEST	Ampicillin
CB-DNA-0019	human	RPA2				BirA-Flag	none	pcDNA5/FRT/DEST	Ampicillin
CB-DNA-0020	human	RPA3				BirA-Flag	none	pcDNA5/FRT/DEST	Ampicillin
CB-DNA-0021	human	PRIM1				BirA-Flag	none	pcDNA5/FRT/DEST	Ampicillin
CB-DNA-0022	human	SLFN11				EGFP	none	pcDNA3/DOriSV40	Ampicillin
CB-DNA-0023	human	SLFN11			K605M+D668A	EGFP	none	pcDNA3/DOriSV40	Ampicillin
CB-DNA-0024	human	SLFN11				none	none	pLVX	Ampicillin
CB-DNA-0025	human	BRCA4				NLS	Flag	pcDNA3/DOriSV40	Ampicillin
CB-DNA-0026	human	SMARCAL1				EGFP	none	pcDNA3/DOriSV40	Ampicillin
CB-DNA-0027	human	SMARCAL1			R764Q	EGFP	none	pcDNA3/DOriSV40	Ampicillin
CB-DNA-0028	human	ZRANB3	X2 variant			EGFP	none	pcDNA3/DOriSV40	Ampicillin
CB-DNA-0029	human	ZRANB3	isoform 3			EGFP	none	pcDNA3/DOriSV40	Ampicillin
CB-DNA-0030	human	ZRANB3	isoform 1			EGFP	none	pcDNA3/DOriSV40	Ampicillin
CB-DNA-0031	human	ZRANB3	isoform 1		K65R	EGFP	none	pcDNA3/DOriSV40	Ampicillin
CB-DNA-0032	human	WRNIP1	isoform 1			EGFP	none	pcDNA3/DOriSV40	Ampicillin
CB-DNA-0033	human	WRNIP1	x2 variant			EGFP	none	pcDNA3/DOriSV40	Ampicillin
CB-DNA-0034	human	WRNIP1	isoform 1		T294A	EGFP	none	pcDNA3/DOriSV40	Ampicillin
CB-DNA-0035	human	HLTF	isoform 1		D557A+E558A	EGFP	none	pcDNA3/DOriSV40	Ampicillin
CB-DNA-0036	human	NRF2				none	none	pcDNA3	Ampicillin
CB-DNA-0037	human	CHIP				EGFP	none	pcDNA3/DOriSV40	Ampicillin
CB-DNA-0038	human	CHIP			K30A	EGFP	none	pcDNA3/DOriSV40	Ampicillin
CB-DNA-0039	human	MC2R				Myc	none	pcDNA5/FRT	Ampicillin	available in a bicistronic vector with MRAPalpha or beta| T143 and N-glycosylation mutants are available
CB-DNA-0040	human	MRAP1	MRAPa				Flag	pcDNA5/FRT	Ampicillin	many tagged versions available
CB-DNA-0041	human	MRAP1	MRAPb				Flag	pcDNA5/FRT	Ampicillin	many tagged versions available
CB-DNA-0056	human	MRAP2					Flag	pcDNA5/FRT	Ampicillin
CB-DNA-0042	human	CHD2	long isoform			HA	none	pCS2	Ampicillin	Stabilized DNA sequence --> doesn't recombine.
CB-DNA-0043	human	TWIST1					none	pEASY-Blunt3	Ampicillin	2,1 kb genomic region containing exon 1 and 2
CB-DNA-0044	human	PTPRZ1		5803			mCherry	pCDH-EF1a-RedLuc | pGK	Ampicillin
CB-DNA-0045	human	PTPRZ1		5803			mOrange2	pCDH-EF1a-RedLuc | pGK	Ampicillin
CB-DNA-0046	human	CDK6		1021			mTurquoise2	pCDH-EF1a-RedLuc | pGK	Ampicillin
CB-DNA-0047	human	DBF4B	isoform 2	80174			mScarlet-I	pCDH-EF1a-RedLuc | pGK	Ampicillin
CB-DNA-0048	human	DBF4B	isoform 1	80174				pCDH-EF1a-RedLuc | pGK	Ampicillin
CB-DNA-0049	human	EZH2		2146			CreiLOV	pCDH-EF1a-RedLuc | pGK	Ampicillin
CB-DNA-0050	human	MCM7	isoform 1	4176			mKate2	pCDH-EF1a-RedLuc | pGK	Ampicillin
CB-DNA-0051	human	VGF1		7425			mBlueberry2	pCDH-EF1a-RedLuc | pGK	Ampicillin
CB-DNA-0052	human	XRCC2		7516			mGarnett2	pCDH-EF1a-RedLuc | pGK	Ampicillin
CB-DNA-0053	human	AKT1			T308A-S473A		turboGFP		Ampicillin
CB-DNA-0054	human	AKT1			T308D-S473D		turboGFP		Ampicillin
CB-DNA-0055	human	AKT1					turboGFP		Ampicillin

Available cDNAs as of 2019-05-16.