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DNA Cloning Service in Expression Vector – CB-CLONE

250.00$660.00$ CAD

This DNA Cloning Service provided by Civic Bioscience is for cloning any sequence into an expression vector of your choice (provided by yourself).

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SKU: CB-CLONE Category: Tags: , , , Brand:

Description

DNA Cloning Service for Expression Vectors

This cloning service provided by Civic Bioscience is for cloning any sequence into an expression vector of your choice (provided by yourself) or one of TransGen Biotech‘s pEASY Expression Vectors.


Pricing Cost of CivicBio's DNA Cloning Services

DNA %GC / kb< 1 kb1-2 kb2-3 kb3-4 kb4-5 kb> 5 kb
20-30 % GC320 $360 $400 $440 $610 $610 $* + 400$/extra kb
31-40 % GC280 $320 $360 $400 $550 $550 $* + 250$/extra kb
41-60 % GC250 $290 $330 $370 $500 $500 $* + 150$/extra kb
61-70 % GC280 $320 $360 $400 $550 $550 $* + 200$/extra kb
71-80 % GC310 $350 $410 $460 $610 $610 $* + 350$/extra kb
81-85 % GC350 $400 $450 $530 $750 $750 $* + 500$/extra kb
86-90 % GC400 $*460 $*560 $*650 $*900 $*900 $* + 750$/extra kb
Estimative pricing of DNA cloning service costs depending on GC percentage and DNA lenght. Actual pricing may vary depending on factors such as the presence of repetitive sequences, the destination vector, toxicity and required competent cells. A 30$ extra charge may apply for the use of specialized competent cells. An extra charge is also applied in the event that the cloned DNA consists of a gene fusion (50$/internal junction).
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Rare DNA clones available from CivicBio | 2019-05-16

Cat. No.SpeciesGOIIsoformGene IDMutationN-ter tagC-ter tagVector 1Antibiotic RNote(s)
CB-DNA-0001humanRAD51Q313K3xFlagnonepCMVKananmycin
CB-DNA-0002humanPOLA2EGFPnonepcDNA3/DOriSV40Ampicillin
CB-DNA-0003humanPRIM2EGFPnonepcDNA3/DOriSV40Ampicillin
CB-DNA-0004humanCXorf57X1EGFPnonepcDNA3/DOriSV40Ampicillin
CB-DNA-0004humanCXorf57X2EGFPnonepcDNA3/DOriSV40Ampicillin
CB-DNA-0005humanCXorf57X1nonenonepLVXAmpicillin
CB-DNA-0006humanRAD51EGFPnonepcDNA3/DOriSV40Ampicillin
CB-DNA-0007humanICE2EGFPnonepcDNA3/DOriSV40Ampicillin
CB-DNA-0008humanBLMK695AEGFPnonepCMV/DOriSV40Kananmycin
CB-DNA-0009humanBLMEGFPnonepCMV/DOriSV40Kananmycin
CB-DNA-0010humanBLMK695AEGFPnonepCMVKananmycin
CB-DNA-0011humanBLMEGFPnonepCMVKananmycin
CB-DNA-0012humanPOLA1EGFPnonepcDNA3/DOriSV40Ampicillin
CB-DNA-0013humanRPA1EGFPnonepcDNA3/DOriSV40Ampicillin
CB-DNA-0014humanRPA2EGFPnonepcDNA3/DOriSV40Ampicillin
CB-DNA-0015humanRPA3EGFPnonepcDNA3/DOriSV40Ampicillin
CB-DNA-0016humanPRIM1EGFPnonepcDNA3/DOriSV40Ampicillin
CB-DNA-0017humanPOLA1BirA-FlagnonepcDNA5/FRT/DESTAmpicillinStabilized DNA sequence --> doesn't recombine. Ultra-low DNA yield.
CB-DNA-0018humanRPA1BirA-FlagnonepcDNA5/FRT/DESTAmpicillin
CB-DNA-0019humanRPA2BirA-FlagnonepcDNA5/FRT/DESTAmpicillin
CB-DNA-0020humanRPA3BirA-FlagnonepcDNA5/FRT/DESTAmpicillin
CB-DNA-0021humanPRIM1BirA-FlagnonepcDNA5/FRT/DESTAmpicillin
CB-DNA-0022humanSLFN11EGFPnonepcDNA3/DOriSV40Ampicillin
CB-DNA-0023humanSLFN11K605M+D668AEGFPnonepcDNA3/DOriSV40Ampicillin
CB-DNA-0024humanSLFN11nonenonepLVXAmpicillin
CB-DNA-0025humanBRCA4NLSFlagpcDNA3/DOriSV40Ampicillin
CB-DNA-0026humanSMARCAL1EGFPnonepcDNA3/DOriSV40Ampicillin
CB-DNA-0027humanSMARCAL1R764QEGFPnonepcDNA3/DOriSV40Ampicillin
CB-DNA-0028humanZRANB3X2 variantEGFPnonepcDNA3/DOriSV40Ampicillin
CB-DNA-0029humanZRANB3isoform 3EGFPnonepcDNA3/DOriSV40Ampicillin
CB-DNA-0030humanZRANB3isoform 1EGFPnonepcDNA3/DOriSV40Ampicillin
CB-DNA-0031humanZRANB3isoform 1K65REGFPnonepcDNA3/DOriSV40Ampicillin
CB-DNA-0032humanWRNIP1isoform 1EGFPnonepcDNA3/DOriSV40Ampicillin
CB-DNA-0033humanWRNIP1x2 variantEGFPnonepcDNA3/DOriSV40Ampicillin
CB-DNA-0034humanWRNIP1isoform 1T294AEGFPnonepcDNA3/DOriSV40Ampicillin
CB-DNA-0035humanHLTFisoform 1D557A+E558AEGFPnonepcDNA3/DOriSV40Ampicillin
CB-DNA-0036humanNRF2nonenonepcDNA3Ampicillin
CB-DNA-0037humanCHIPEGFPnonepcDNA3/DOriSV40Ampicillin
CB-DNA-0038humanCHIPK30AEGFPnonepcDNA3/DOriSV40Ampicillin
CB-DNA-0039humanMC2RMycnonepcDNA5/FRTAmpicillinavailable in a bicistronic vector with MRAPalpha or beta| T143 and N-glycosylation mutants are available
CB-DNA-0040humanMRAP1MRAPaFlagpcDNA5/FRTAmpicillinmany tagged versions available
CB-DNA-0041humanMRAP1MRAPbFlagpcDNA5/FRTAmpicillinmany tagged versions available
CB-DNA-0056humanMRAP2FlagpcDNA5/FRTAmpicillin
CB-DNA-0042humanCHD2long isoformHAnonepCS2AmpicillinStabilized DNA sequence --> doesn't recombine.
CB-DNA-0043humanTWIST1nonepEASY-Blunt3Ampicillin2,1 kb genomic region containing exon 1 and 2
CB-DNA-0044humanPTPRZ15803mCherrypCDH-EF1a-RedLuc | pGKAmpicillin
CB-DNA-0045humanPTPRZ15803mOrange2pCDH-EF1a-RedLuc | pGKAmpicillin
CB-DNA-0046humanCDK61021mTurquoise2pCDH-EF1a-RedLuc | pGKAmpicillin
CB-DNA-0047humanDBF4Bisoform 280174mScarlet-IpCDH-EF1a-RedLuc | pGKAmpicillin
CB-DNA-0048humanDBF4Bisoform 180174pCDH-EF1a-RedLuc | pGKAmpicillin
CB-DNA-0049humanEZH22146CreiLOVpCDH-EF1a-RedLuc | pGKAmpicillin
CB-DNA-0050humanMCM7isoform 14176mKate2pCDH-EF1a-RedLuc | pGKAmpicillin
CB-DNA-0051humanVGF17425mBlueberry2pCDH-EF1a-RedLuc | pGKAmpicillin
CB-DNA-0052humanXRCC27516mGarnett2pCDH-EF1a-RedLuc | pGKAmpicillin
CB-DNA-0053humanAKT1T308A-S473AturboGFPAmpicillin
CB-DNA-0054humanAKT1T308D-S473DturboGFPAmpicillin
CB-DNA-0055humanAKT1turboGFPAmpicillin
Available cDNAs as of 2019-05-16.

Additional information

DNA insert

< 1 kb, < 2 kb, < 3 kb, < 4 kb, < 5 kb, < 6 kb

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