TransStart KD Plus DNA Polymerase (Ultra-HiFi) – AP301
114.00$ – 1,047.00$CAD
KD Plus DNA Polymerase (Ultra-High-Fidelity)
TransStart®KD Plus DNA Polymerase is a genetically modified ultra-high fidelity DNA polymerase in hotstart formulation. This enzyme provides higher amplification capability than traditional Pfu DNA polymerase and amplification speed equal to Taq DNA polymerase (1 kb/min). Due to strong 3’-5’ exonuclease activity, this enzyme offers 108-fold fidelity as compared toEasyTaq® DNA Polymerase. It also benefits from the TransStart® double Hot start technology.
NOTE from Civic Bioscience : In our hands, KD Plus is the High-Fidelity DNA Polymerase of choice for AT-rich PCR that often requires an extension temperature of 62-68°C.
Advantages of TransStart® KD Plus DNA Polymerase
- PCR product carries blunt ends, which can be directly cloned into pEASY®-Blunt vectors.
- It’s moderate amplification speed (1 kb/min) allows for better amplification of GC-rich and AT-rich templates
- Its ultra high proofreading capacity makes this polymerase ideal for cloning, library amplification and other application requiring excellent fidelity.
- Amplification of genomic DNA fragment up to 15 kb.
- Amplification of plasmid DNA fragment up to 20 kb.
- Available with or without 2.5 mM dNTPs (1 ml) for additional convenience.
Manual and datasheet
Additional information
| Format | 6 x 200 u, 6 x 200 u + dNTPs, 100 u, 100 u + dNTPs, 200 u, 200 u + dNTPs |
|---|---|
| Supplier |
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Reviews for TransStart KD Plus DNA Polymerase (Ultra-HiFi) – AP301
4 reviews for TransStart KD Plus DNA Polymerase (Ultra-HiFi) - AP301
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srcivicbio –
User comment and results submitted by email from a customer at Université de Montréal. For privacy reasons, the amplified genes are not specified.
Voici le gel dont je t’avais parlé. La piste 8 est la plus révélatrice. Tu peux la comparer aux autres enzymes 6,7 et 9.
>Gene : 8.4kbp
GC% : 70%
Pistes :
1. MW
2.à 5. c’était des tests avec une ADN de départ avec GC 30%, même enzymes mais différents primers.
6. FastPfu Fly
7. FastPfu
8. KD Plus
9. Q5 HS
Attachment
Simon Roy (verified owner)–
Comment received by email from the University of British Columbia:
I succeeded amplifying a 2.5k fragment with KD PLUS and trying to amplify another fragment. Before that, I tried FastPfu but it seems like KD Plus is optimal for my case.
Dominique Lévesque (verified owner)–
one of the most powerful polymerase I ever used. I recommend!!! five stars!!!
Phil Roche –
Using the Pre-mix Kdplus we’ve been very happy with it’s performance in GC rich amplifications. Good yield with reliable, high fidelity product.