TransStart KD Plus (Ultra-HiFi) DNA Polymerase – AP301

TransStart KD Plus (Ultra-HiFi) DNA Polymerase

TransStart® KD Plus DNA Polymerase is a genetically modified ultra-high fidelity DNA polymerase in hotstart formulation. This enzyme provides higher amplification capability than traditional Pfu DNA polymerase and amplification speed equal to Taq DNA polymerase (1 kb/min). Due to strong 3’-5’ exonuclease activity, this enzyme offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase. It also benefits from the TransStart® double Hot start technology.

NOTE from Civic Bioscience : In our hands, KD Plus is the High-Fidelity DNA Polymerase of choice for AT-rich PCR that often requires an extension temperature of 62-68°C.

Advantages of TransStart® KD Plus DNA Polymerase

• PCR product carries blunt ends, which can be directly cloned into pEASY®-Blunt vectors.
• It’s moderate amplification speed (1 kb/min) allows for better amplification of GC-rich and AT-rich templates
• Its ultra high proofreading capacity makes this polymerase ideal for cloning, library amplification and other application requiring excellent fidelity.
• Amplification of genomic DNA fragment up to 15 kb.
• Amplification of plasmid DNA fragment up to 20 kb.
• Available with or without 2.5 mM dNTPs (1 ml) for additional convenience.

Manual and datasheet

AP301 TransStart® KD Plus DNA Polymerase