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TransStart KD Plus DNA Polymerase (Ultra-HiFi) – AP301

(4 customer reviews)

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KD Plus DNA Polymerase (Ultra-High-Fidelity)

TransStart®KD Plus DNA Polymerase is a genetically modified ultra-high fidelity DNA polymerase in hotstart formulation. This enzyme provides higher amplification capability than traditional Pfu DNA polymerase and amplification speed equal to Taq DNA polymerase (1 kb/min). Due to strong 3’-5’ exonuclease activity, this enzyme offers 108-fold fidelity as compared toEasyTaq® DNA Polymerase. It also benefits from the TransStart® double Hot start technology.

NOTE from Civic Bioscience : In our hands, KD Plus is the High-Fidelity DNA Polymerase of choice for AT-rich PCR that often requires an extension temperature of 62-68°C.


Advantages of TransStart® KD Plus DNA Polymerase

  • PCR product carries blunt ends, which can be directly cloned into pEASY®-Blunt vectors.
  • It’s moderate amplification speed (1 kb/min) allows for better amplification of GC-rich and AT-rich templates
  • Its ultra high proofreading capacity makes this polymerase ideal for cloning, library amplification and other application requiring excellent fidelity.
  • Amplification of genomic DNA fragment up to 15 kb.
  • Amplification of plasmid DNA fragment up to 20 kb.
  • Available with or without 2.5 mM dNTPs (1 ml) for additional convenience.

TGAP301 contentTGAP301 gel


Manual and datasheet

AP301 TransStart® KD Plus DNA Polymerase

Additional information

Format

6 x 200 u, 6 x 200 u + dNTPs, 100 u, 100 u + dNTPs, 200 u, 200 u + dNTPs

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Reviews for TransStart KD Plus DNA Polymerase (Ultra-HiFi) – AP301

4 reviews for TransStart KD Plus DNA Polymerase (Ultra-HiFi) - AP301

  1. srcivicbio

    User comment and results submitted by email from a customer at Université de Montréal. For privacy reasons, the amplified genes are not specified.

    Voici le gel dont je t’avais parlé. La piste 8 est la plus révélatrice. Tu peux la comparer aux autres enzymes 6,7 et 9.

    >Gene : 8.4kbp
    GC% : 70%
    Pistes :
    1. MW
    2.à 5. c’était des tests avec une ADN de départ avec GC 30%, même enzymes mais différents primers.
    6. FastPfu Fly
    7. FastPfu
    8. KD Plus
    9. Q5 HS

    Attachment KD Plus amplification of GC-rich template

  2. Simon Roy (verified owner)

    Comment received by email from the University of British Columbia:

    I succeeded amplifying a 2.5k fragment with KD PLUS and trying to amplify another fragment. Before that, I tried FastPfu but it seems like KD Plus is optimal for my case.

  3. Dominique Lévesque (verified owner)

    one of the most powerful polymerase I ever used. I recommend!!! five stars!!!

  4. Phil Roche

    Using the Pre-mix Kdplus we’ve been very happy with it’s performance in GC rich amplifications. Good yield with reliable, high fidelity product.

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