King Kong Pfu DNA Polymerase (Ultra-High Fidelity) – CB-KP231

King Kong Pfu Ultra-High Fidelity DNA Polymerase

King Kong Pfu (KKP) Ultra-High Fidelity (U-HF) DNA Polymerase has a very low error rate despite its high-processivity. KKP U-HF was engineered for all-around PCR performance that provide for increased accuracy, permissivity, conveniency and versatility on top of being formulated in a hassle-free hotstart formulation.

KKP consists is a modified Pfu DNA Polymerase and behaves as such with regards to PCR setup and cycling conditions. The amplicons generated by King Kong Pfu are blunt-ended. The PCR products perform particuliarly well in seamless DNA assembly methods, mutagenesis, amplicon fusion and overall cloning workflows, including blunt-end topoisomerase-based 5 min cloning kits.

The recommended annealing temperature when using King King Pfu DNA Polymerase in standard PCR ampification is that which is provided by the Snapgene software.

Read more:

• Learn all about FLY
• GC-rich PCR competitor comparison.
• GC-rich PCR competitor comparison – PCR Success Story #21

Advantages of King Kong Pfu DNA Polymerase in PCR

• Fidelity is 108 times higher than Taq Wondo Taq DNA Polymerase.
• HotStart : Extremely specific amplification.
• The extension rate is 2-6 kb/min.
• PCR products can be directly cloned into TOPO-Blunt vectors.
• Amplification of genomic DNA fragment up to 15 kb.
• Amplification of plasmid DNA fragment up to 20 kb.

Manual and datasheet

King Kong Pfu Ultra-High Fidelity DNA Polymerase v1 2023-01-18

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