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CivicBio PCR Success Stories & PCR Services

Cloning, Site-Directed Mutagenesis, PCR, Genotyping by PCR
Civic Bio provides FREE and contractual PCR Services to researchers and companies. You can browse from our completed PCR projects on this page, also known as PCR Success Stories!
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Completed PCR Projects – PCR Success Stories

PCR Services: cloning, site-directed mutagenesis, mouse genotyping and others.

Success #24 : STR analysis and Prenatal Sex Genotyping by PCR from Fetal Cell-Free DNA

Success #24 : STR analysis and Prenatal Sex Genotyping by PCR from Fetal Cell-Free DNA

Success #23: A better multiplex PCR protocol for detecting WT and KO alleles from Dusp4 / Mkp2 knockout mice

Success #23: A better multiplex PCR protocol for detecting WT and KO alleles from Dusp4 / Mkp2 knockout mice

Success #22: Site-Directed Mutagenesis on a Minicircle using Overlap Extension PCR

Success #22: Site-Directed Mutagenesis on a Minicircle using Overlap Extension PCR

Success #21: GC-Rich Cloning of HLA Nonclassical Class I Heavy Chain

Success #21: GC-Rich Cloning of HLA Nonclassical Class I Heavy Chain

Success #20: AT-Rich Vector Amplification by Fast & Steep PCR @ ULaval

Success #20: AT-Rich Vector Amplification by Fast & Steep PCR @ ULaval

Success #19: Insertion of Stop Codons by Fast & Steep PCR Site-Directed Mutagenesis and Seamless Assembly

Success #19: Insertion of Stop Codons by Fast & Steep PCR Site-Directed Mutagenesis and Seamless Assembly

Success #18: 16S rRNA gene identification @ Private company

Success #18: 16S rRNA gene identification @ Private company

Success #17: PCR Overlap Assembly of 3 Fragments @ ULaval

Success #17: PCR Overlap Assembly of 3 Fragments @ ULaval

Success #16: Site-Directed Mutagenesis by PCR Overlap and Fast Mutagenesis Techniques

Success #16: Site-Directed Mutagenesis by PCR Overlap and Fast Mutagenesis Techniques

Success #15: Complete Design, Cloning and Truncation Analysis of a Human Promoter

Success #15: Complete Design, Cloning and Truncation Analysis of a Human Promoter

Success #14: High-Fidelity PCR Amplification of Pseudomonas aeruginosa murC and murE

Success #14: High-Fidelity PCR Amplification of Pseudomonas aeruginosa murC and murE

Success #13: Mouse Genotyping by PCR of a Large Colony

Success #13: Mouse Genotyping by PCR of a Large Colony

Success #12: Extreme GC Content Site-Directed Mutagenesis

Success #12: Extreme GC Content Site-Directed Mutagenesis

Success #11: 10xHis Tag Insertion by Site-Directed Mutagenesis

Success #11: 10xHis Tag Insertion by Site-Directed Mutagenesis

Success #10: MC4R Mouse Genotyping by PCR

Success #10: MC4R Mouse Genotyping by PCR

Success #9: High-Fidelity & Specific PCR from Human gDNA

Success #9: High-Fidelity & Specific PCR from Human gDNA

Success #8: Human gDNA Quality Control

Success #8: Human gDNA Quality Control

Success #7: Tph2 Mouse Genotyping by PCR

Success #7: Tph2 Mouse Genotyping by PCR

Success #6: Cre and A2A Mouse Genotyping by PCR

Success #6: Cre and A2A Mouse Genotyping by PCR

Success #5: Difficult PCR Amplification of CHD2 (long isoform) from human cDNA

Success #5: Difficult PCR Amplification of CHD2 (long isoform) from human cDNA

Success #4: Cloning from cDNA: human SLUG, VSP24 and NKCC2

Success #4: Cloning from cDNA: human SLUG, VSP24 and NKCC2

Success #3: DsRed Mouse Genotyping by PCR

Success #3: DsRed Mouse Genotyping by PCR

Success #2: Mouse DNA genotyping by PCR at the Lady Davis Institute

Success #2: Mouse DNA genotyping by PCR at the Lady Davis Institute

Success #1: EGFP, flox/src, Cre, Confetti Genotyping by PCR

Success #1: EGFP, flox/src, Cre, Confetti Genotyping by PCR

What Our Customers Are Saying about our PCR Services

Don’t just take it from us, let our customers do the talking!

Bon… j’ai rĂ©ussi 🙂
Le Mutliplex fonctionne.
Je peux désormais, grâce à ton expertise, génotyper mes A2AFlox, mes Ucp1Cre et mes AdipoQCre avec le même mastermix!
Merci beaucoup!
T’es un chef!
L

Technicienne, CR-CHUM

Allo Simon,

Je viens de placer une commande de l’enzyme EasyTaq. Merci beaucoup,

Tu as rĂ©glĂ© un problème rĂ©current que j’avais avec cette paire d’amorces.

M

Assistante de Recherche, University of Ottawa

La Fly est super! On a eu 3 gènes avec la KD et le dernier on a essayé la FLY ça crache!
C

Chercheure, Université de Montréal

Salut Simon

J’ai besoin de commander de la FastPfu Fly. La Fly est parfaite. Elle va rĂ©gler tous nos problèmes.

Si je prends un kit

EasyPure® Blood Genomic DNA Kit (with Proteinase K) 50 Rx

et

TransStart® FastPfu FLY (Ultra-HiFi) DNA Polymerase 500 Rx

Peux-tu me faire une soumission?


PCR Success Story #9

M

Assistant de Recherche, CHU Ste-Justine

Je viens de faire mon PCR Ă  55 et 57, 10 sec j’ai une belle bande unique avec la EasyTaq. merci tout plein pour le troubleshooting.
A

Assistante de recherche, Lady Davis Institute

Bon, tu peux te péter les bretelles encore une fois!
Ta Easytaq est malade!!! Il me faut tous les détails de ton protocole et ton # de catalogue pour la Taq ?

Check ça et devine quelle ligne est ta Taq. exposition ok pour ta TAQ et disparition de Lucigen!!!

Je n’ai jamais eu d’aussi belles bandes qu’avec ta EasyTaq!


PCR Success Story #8

M

Assistante de recherche, CR-CHUM

Salut Simon,

C’est excellent… bien meilleur que ce que j’obtenais.

Merci pour la mutagĂ©nèse, je te prends la FastPfu et la FLY et j’ai dĂ©jĂ  la KD Plus.

Y

Assistant de Recherche, Université de Montréal

Allo Simon,

J’ai fait le tour de la plupart de mes PCRs avec ta EasyTaq et mon constat c’est qu’elle marche très bien mĂ«me avec certains PCRs plus difficiles.

Ceci dit, je vais continuer d’inciter les gens du lab a l’utiliser en remplacement des autres taqs que nous avons.

Bonne journee!


PCR Success Story #1

C

Assistante de recherche, McGill University

Allo Simon,

J’ai fait un PCR avec ta FastPfu hier, ça fonctionne bien. Reste Ă  savoir si on peut le transcrire. Ça sert pas Ă  grand chose que tu fasses des tests car le problème est rĂ©solu. … je passerai une commande quand j’aurai besoin de phusion!

Merci,

A-M

Assistante de recherche, Université de Sherbrooke

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