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Focus on Results with our PCR Services


PCR Services from Civic Bio

 

Mouse Genotyping by PCR

Cloning from cDNA, gDNA or other

Site-Directed Mutagenesis

16s ribosomal RN

PCR Problems ?


FREE PCR Service – We solve PCR problems!

  • Difficult cloning (AT-rich or GC-rich PCR)
  • Genotyping
  • Others

Our PCR Optimization Service is FREE  when you ship us your samples and oligos.

We can also arrange shipping for 25$.

Custom strategy

Complete Custom Experimental Design

We design everything for you. Tailored to your needs. No need to worry about feasability.

Snapgene data

Plasmid Maps

Complete and detailed maps provided in Snapgene format, Genbank or Fasta.

Sequencing Results

Sequencing Results

DNA sequencing is performed at the Plateforme de Séquençage du CHUL in Quebec City.

Glycerol Stocks

Glycerol Stocks

In contrast to other service providers, we provide the bacteria glycerol stocks upon project completion. No need to perform plasmid DNA transformation on reception. Whenever possible, plasmid DNA is maintained in T1-phage and T5-phage resistant competent cells.

Fair Pricing

Fair Pricing

Discounts and/or free complementary products are offered for large projects or long-term projects.

En utilisant votre service de clonage et de mutagénèse, je peux me concentrer les les expériences qui sont vraiment importantes pour le laboratoire afin d’accélérer la vitesse de nos publications. En plus, le travail est garanti.

David

Researcg Assistant, McGill

Si j’ai à cloner ou faire de la mutagénèse, simplement le coût d’acheter 2 enzymes et des bactéries coûte plus cher et le travail n’est toujours pas fait. Je préfère largement utiliser un service de clonage et avancer dans mes expériences durant ce temps.

Alex

Ph.D Student, UOttawa

Focus on results

Focus on Results, not on building tools to generate results.

Plans and Pricing

As customized solutions may require personnalized pricing, we offer you a first look at what our PCR service pricing looks like. Please remember that PCR Service pricing may vary according to your needs and demands.

Genotyping by PCR

DNA extraction not included
4 $/sample (48 or less)
  • Personnalized solution
  •  
  • Agarose gel data
  • Excel spreadsheet recapitulation of PCR results
  • Primers are not provided (6$ each)
  • DNA exraction not included: 6$ per sample for 48 samples or less.
  • 25$ sample shipping fee

Cloning from cDNA

in expression vector
250 $/sample (3 or less)
  • Vector map and sequence verified by sequencing
  •  
  • 10 ug of pure plasmid DNA provided & Glycerol stock(s)
  • Custom design tailored to your needs (facultative)
  • Choice of any pEASY vector or your provided vector
  • Sample shipping fee : 25$
  • User provided gDNA or cDNA; cost of primers may be extra if > 35 bp

PCR Service Quote Request Form

What you need

What Our Customers Are Saying about our PCR Service

Don’t just take it from us, let our customers do the talking!

Bon… j’ai réussi 🙂
Le Mutliplex fonctionne.
Je peux désormais, grâce à ton expertise, génotyper mes A2AFlox, mes Ucp1Cre et mes AdipoQCre avec le même mastermix!
Merci beaucoup!
T’es un chef!

L

Technicienne, CR-CHUM

Allo Simon,

Je viens de placer une commande de l’enzyme EasyTaq.  Merci beaucoup,

Tu as réglé un problème récurrent que j’avais avec cette paire d’amorces.

M

Assistante de Recherche, University of Ottawa

Salut Simon

J’ai besoin de commander de la FastPfu Fly. La Fly est parfaite. Elle va régler tous nos problèmes.

 


PCR Success Story #9

M

Assistant de Recherche, CHU Ste-Justine

Je viens de faire mon PCR à 55 et 57, 10 sec j’ai une belle bande unique avec la EasyTaq. merci tout plein pour le troubleshooting.

A

Assistante de recherche, Lady Davis Institute

Bon, tu peux te péter les bretelles encore une fois!
Ta Easytaq est malade!!! Il me faut tous les détails de ton protocole et ton # de catalogue pour la Taq ?

Je n’ai jamais eu d’aussi belles bandes qu’avec ta EasyTaq!


PCR Success Story #8

M

Assistante de recherche, CR-CHUM

Salut Simon,

C’est excellent… bien meilleur que ce que j’obtenais.

Merci pour la mutagénèse, je te prends la FastPfu et la FLY et j’ai déjà la KD Plus.

Y

Assistant de Recherche, Université de Montréal

Frequently Asked Questions

Why is your pricing so low for mouse genotyping by PCR? Other service providers charge 10$ per PCR reaction!

Good question. Because we distribute high-quality DNA polymerases and extraction kits from diverse suppliers, this reduces our cost for performing these experiments. As simple as that!

What do I need to buy to use your free PCR optimization service?

Our PCR optimization service is free.

Upon reception of our results we will show you the successful results and the PCR setup recipe but not the cycling protocol.

The logical next step however is for you to repeat our experiment. To do such a thing you will likely have to purchase one of our DNA polymerases. DNA polymerases pricing starts at 30$.

Can you clone or perform site-directed mutagenesis on GC- or AT-rich templates?

Yes we can.

As we have many DNA polymerases to choose from, the likelyhood of being successful at amplifying GC- or AT-rich templates is high. However, some templates may be more problematic and may require a longer lead time. So far we have achieved 80% and 27% GC content amplifications.

Such projects may be subjected to different pricing.

If we are not successful, we give you your money back.

What fragment size can you clone?

We can achieve cloning of basically any cDNA < 10 kb.

As difficulty increases and sequencing costs increase for cloning of DNA fragments > 1.5 kb, our pricing will also increase for this type of cloning service. Please request a quote.

Don't Be Shy

If we didn’t answer all of your questions, feel free to drop us a line anytime about our PCR Service or anything else!