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TransStart® Taq DNA Polymerase – AP141

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TransStart® Taq DNA Polymerase

TransStart® Taq DNA Polymerase is a hot start Taq DNA polymerase containing Taq DNA polymerase and two proprietary DNA binding proteins. At room temperature, one binding protein binds to doublestranded DNA template and another binding protein binds to primers. These unique formulations effectively neutralize the DNA polymerase activity at room temperature. Blocking proteins are released from primers and templates during the initial denaturation. This double blocking method has higher efficiency than antibody based, or chemically modified hot start PCR.


Advantages TransStart® Taq DNA Polymerase

  • Fidelity is 18 times higher than EasyTaq® DNA Polymerase.
  • The extension rate is about 1-2 kb/min.
  • Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
  • Reduced nonspecific amplification and primer dimer formation.
  • Different from Taq antibody, no risk of contamination from mammal DNA.
  • Different from chemical modification, long time predenatured step is no longer needed.
  • Excellent choice for homebrewed qPCR mixes.
  • Amplification of genomic DNA fragment up to 15 kb.
  • Available with or without 2.5 mM dNTPs for additional convenience.

TGAP141 content

TGAP141 gel


Manual and Datasheet

AP141 TransStart® Taq DNA Polymerase


TransStart Hot Start Technology

TransStart double blocking technology

Additional information

Format

6 x 500u, 6 x 500u + dNTPs, 250u + dNTPs, 250 u, 500u + dNTPs, 500 u

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Published on Jan 30, 2017 by Simon Roy, Ph.D Detection Limit of High-Fidelity DNA Polymerases using Human gDNA for Template We previously determined the detection limit of different Taq DNA Polymerases using human genomic DNA as a template for PCR. For this instance,...

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86.00$872.00$ CAD

Clear

Cashback Reward : Earn up to 87$!