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TransStart FastPfu High-Fidelity DNA Polymerase – AP221

(2 customer reviews)

119.00$1,026.00$ CAD

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FastPfu High-Fidelity DNA Polymerase

TransStart FastPfu High-Fidelity DNA Polymerase is a fast, high-fidelity and high-processivity DNA polymerase combined to double-hotstart (TransStart®) technology. It consists in a modified Pfu Polymerase increasing its affinity for dsDNA and enhancing processivity and other caracteristics.

Comparison with other high-fidelity DNA Polymerases


Advantages of FastPfu DNA Polymerase

  • The extension rate is about 2-4 kb/min.
  • Fidelity is 54 times higher than EasyTaq DNA Polymerase.
  • Blunt-end PCR products can be directly cloned into pEASY®-Blunt vectors.
  • Amplification of genomic DNA fragment up to 15 kb.
  • Amplification of plasmid DNA fragment up to 20 kb.
  • Available with or without 2.5 mM dNTPs for additional convenience.

TGAP221 contentTGAP221 suggestionTGAP221 gel1TGAP221 gel2

 


Manual and datasheet

AP221 TransStart FastPfu DNA Polymerase

Additional information

Format

6 x 500u, 6 x 500u + dNTPs, 250u, 250u + dNTPs, 500u, 500u + dNTPs

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DNA Polymerase Comparison Chart

  Specifications and pricing of commercially available Taq DNA Polymerases and High-Fidelity DNA Polymerases in Canada.      

Detection Limit of High-Fidelity DNA Polymerases on gDNA

Published on Jan 30, 2017 by Simon Roy, Ph.D Detection Limit of High-Fidelity DNA Polymerases using Human gDNA for Template We previously determined the detection limit of different Taq DNA Polymerases using human genomic DNA as a template for PCR. For this instance,...

Product Reviews for TransStart FastPfu High-Fidelity DNA Polymerase – AP221

2 reviews for TransStart FastPfu High-Fidelity DNA Polymerase – AP221

  1. Pat

    La FastPfu torche totalement. oui!…torche! Voici un triple overlap extension PCR en mode “Fast and Steep PCR” (seule et unique tentative) totalisant 5.36kb et d’une moyenne de 66% GC [58; 82%].

    Attachment triple oe-PCR GC rich 5.5kb

  2. Maxime Bouchard (verified owner)

    Very good for amplification when doing cloning

    • Simon Roy (verified owner)

      Merci Mathieu. Your comment will help other scientists.
      Voici ton code promo te donnant droit à CASHBACKx2 pour une commande future: Mathieu AP221

      If you had a chance to add an image, that would be greatly appreciated!
      Cheers

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119.00$1,026.00$ CAD

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