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TransStart FastPfu High-Fidelity DNA Polymerase – AP221

(2 customer reviews)

121.00$1,099.00$ CAD


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FastPfu DNA Polymerase

TransStart FastPfu DNA Polymerase is a fast, high-fidelity and high-processivity DNA polymerase combined to double-hotstart (TransStart®) technology. It was engineered from Pfu Polymerase to increase its affinity for dsDNA and enhance its processivity and other caracteristics.

Comparison with other high-fidelity DNA Polymerases

Advantages of FastPfu DNA Polymerase

  • The extension rate is about 2-4 kb/min.
  • Fidelity is 54 times higher than EasyTaq DNA Polymerase.
  • Blunt-end PCR products can be directly cloned into pEASY®-Blunt vectors.
  • Amplification of genomic DNA fragment up to 15 kb.
  • Amplification of plasmid DNA fragment up to 20 kb.
  • Available with or without 2.5 mM dNTPs for additional convenience.

TGAP221 content TGAP221 suggestion TGAP221 gel1 TGAP221 gel2


Manual and datasheet

AP221 TransStart FastPfu DNA Polymerase

Additional information


6 x 500u, 6 x 500u + dNTPs, 250u, 250u + dNTPs, 500u, 500u + dNTPs


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Reviews for TransStart FastPfu High-Fidelity DNA Polymerase – AP221

2 reviews for TransStart FastPfu High-Fidelity DNA Polymerase - AP221

  1. Maxime Bouchard (verified owner)

    Very good for amplification when doing cloning

    • Simon Roy (verified owner)

      Merci Mathieu. Your comment will help other scientists.
      Voici ton code promo te donnant droit à CASHBACKx2 pour une commande future: Mathieu AP221

      If you had a chance to add an image, that would be greatly appreciated!

  2. Pat

    La FastPfu torche totalement. oui!…torche! Voici un triple overlap extension PCR en mode « Fast and Steep PCR » (seule et unique tentative) totalisant 5.36kb et d’une moyenne de 66% GC [58; 82%].

    Attachment triple oe-PCR GC rich 5.5kb

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