TransTaq HiFi DNA Polymerase – AP131

TransTaq® HiFi DNA Polymerase

TransTaq DNA Polymerase High Fidelity, also named TransTaq HiFi DNA Polymerase, contains TransTaq®-T DNA Polymerase and a second proprietary DNA polymerase possessing proofreading 3’-5’ exonuclease activity. TransTaq HiFi DNA Polymerase provides higher specificity and amplification efficiency than TransTaq®-T DNA Polymerase. It provides two different buffers for optimal PCR conditions: TransTaq® HiFi Buffer I is optimized for the amplification of genomic DNA and TransTaq® HiFi Buffer II is optimized for the amplification of λDNA, cDNA or plasmid DNA.

Advantages of TransTaq HiFi DNA Polymerase

• Fidelity is 18 times higher than EasyTaq® DNA Polymerase.
• The extension rate is about 1-2 kb/min.
• Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Amplification of genomic DNA fragment up to 15 kb.
• Available with or without 1 ml of 2.5 mM dNTPs for convenience.

Manual and datasheet

AP131 TransTaq® DNA Polymerase High Fidelity (HiFi)