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TransTaq HiFi DNA Polymerase – AP131

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TransTaq® HiFi DNA Polymerase

TransTaq DNA Polymerase High Fidelity, also named TransTaq HiFi DNA Polymerase, contains TransTaq®-T DNA Polymerase and a second proprietary DNA polymerase possessing proofreading 3’-5’ exonuclease activity. TransTaq HiFi DNA Polymerase provides higher specificity and amplification efficiency than TransTaq®-T DNA Polymerase. It provides two different buffers for optimal PCR conditions: TransTaq® HiFi Buffer I is optimized for the amplification of genomic DNA and TransTaq® HiFi Buffer II is optimized for the amplification of λDNA, cDNA or plasmid DNA.


Advantages of TransTaq HiFi DNA Polymerase

  • Fidelity is 18 times higher than EasyTaq® DNA Polymerase.
  • The extension rate is about 1-2 kb/min.
  • Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
  • Amplification of genomic DNA fragment up to 15 kb.
  • Available with or without 1 ml of 2.5 mM dNTPs for convenience.

TGAP131 content

TGAP131 gel


Manual and datasheet

AP131 TransTaq® DNA Polymerase High Fidelity (HiFi)

Additional information

Format

6 x 500u, 6 x 500u + dNTPs, 250u + dNTPs, 250 u, 500u + dNTPs, 500 u

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Detection Limit of High-Fidelity DNA Polymerases on gDNA

Detection Limit of High-Fidelity DNA Polymerases on gDNA

Published on Jan 30, 2017 by Simon Roy, Ph.D Detection Limit of High-Fidelity DNA Polymerases using Human gDNA for Template We previously determined the detection limit of different Taq DNA Polymerases using human genomic DNA as a template for PCR. For this instance,...

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68.00$749.00$ CAD

Clear

Cashback Reward : Earn up to 74$!