Detection Limit of High-Fidelity DNA Polymerases using Human gDNA for Template We previously determined the detection limit of different Taq DNA Polymerases using human genomic DNA as a template for PCR. For this instance, we aimed at determining the Detection Limit...
TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix (+oligos) – AT311
261.00$ – 471.00$ CAD
TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix
The TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix combines the unique genomic DNA remover with TransScript® First-Strand cDNA Synthesis SuperMix to achieve simultaneous genomic DNA removal and cDNA synthesis in one tube. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85°C for only 5 seconds.
Advantages of TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix
- Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination.
- After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated simultaneously.
- Compared to traditional pretreatment of RNA with DNase I, this method avoids RNA damage caused by inactivation of DNase I after treatment.
- One-tube simple procedure
- cDNA fragment up to 12 kb
- RNase inhibitor is included.
Manual and Datasheet:
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100 x 20 ul rx, 50 x 20 ul rx
Commercial DNA Polymerase Comparison Chart DNA Polymerase Comparison of different commercially available enzymes with their genral specifications and pricing in Canadian dollars.
Which is the Best Kit for DNA Clean Up? Our aim was to perform a PCR Purification Kit Comparison from different vendors. At Civic Bioscience, we distribute the Favorprep™ and EasyPure® product lines from FAVORGEN and TransBionovo respectively. First, the FavorPrep™...
GC-rich PCR: FastPfu FLY vs Q5® High-Fidelity DNA Polymerase GC-rich PCR can be difficult to achieve. Our aim was to verify compare FastPfu FLY (TransBionovo) and Q5 (NEB) high-fidelity DNA Polymerase at amplifying a GC-rich sequence (human ARX; 79% GC) from purified...