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TransStart FastPfu FLY (Ultra-HiFi) DNA Polymerase – AP231

Rated 5.00 out of 5 based on 2 customer ratings
(2 customer reviews)

Product Description

TransStart FastPfu FLY (Ultra-HiFi) DNA Polymerase

TransStart FastPfu Fly DNA Polymerase is a hotstart, ultra-high fidelity and high-processivity DNA Polymerase. It consists in a modified DNA Polymerase, increasing its affinity for dsDNA and enhancing processivity and other caracteristics. Its fidelity is 108 times higher than EasyTaq DNA polymerase making it the DNA Polymerase with the highest fidelity on the market. In addition, its extension rate reaches 6 kb/min. Compared with FastPfu DNA Polymerase, The FLY version of FastPfu DNA Polymerase has a faster extension rate, higher fidelity, and higher amplification efficiency than regular FastPfu, making it the polymerase of choice for difficult PCR, GC-rich or AT-rich amplicons, library amplification and more.

Advantages of TransStart FastPfu Fly DNA Polymerase AP231

  • Fidelity is 108 times higher than EasyTaq DNA Polymerase.
  • Extremely specific amplification.
  • The extension rate is about 2-6 kb/min.
  • PCR products can be directly cloned into pEASY®Blunt vectors.
  • Amplification of genomic DNA fragment up to 15 kb.
  • Amplification of plasmid DNA fragment up to 20 kb.
  • Available with or without 2.5 mM dNTPs for additional convenience !

TGAP231 content

TGAP231 comparisonTGAP231 data

AP231 FastPfu FLY Fidelity chart

Manual and datasheet:

AP231 TransStart® FastPfu Fly DNA Polymerase

Additional information


250u, 250u + dNTPs, 500u, 500u + dNTPs, 6 x 500u, 6 x 500u + dNTPs

Detection Limit of High-Fidelity DNA Polymerases on gDNA

Detection Limit of High-Fidelity DNA Polymerases using Human gDNA for Template We previously determined the detection limit of different Taq DNA Polymerases using human genomic DNA as a template for PCR. For this instance, we aimed at determining the Detection Limit...

DNA Polymerase Comparison Chart

Commercial DNA Polymerase Comparison Chart DNA Polymerase Comparison of different commercially available enzymes with their genral specifications and pricing in Canadian dollars.

PCR Purification Kit Comparison – DNA Clean Up

Which is the Best Kit for DNA Clean Up? Our aim was to perform a PCR Purification Kit Comparison from different vendors. At Civic Bioscience, we distribute the Favorprep™ and EasyPure® product lines from FAVORGEN and TransBionovo respectively. First, the FavorPrep™...

Product Reviews for TransStart FastPfu FLY (Ultra-HiFi) DNA Polymerase – AP231

2 reviews for TransStart FastPfu FLY (Ultra-HiFi) DNA Polymerase – AP231

  1. Rated 5 out of 5

    Simon Roy (verified owner)

    Commentaire reçu par courriel:

    • Utilisable comme la Veraseq
    • Pas chère
    • Utilisable diluée jusqu’à 1/8X
    • Amplification de long fragments (13kb+)



  2. Rated 5 out of 5


    Customer review submitted by email.
    Agarose gel electrophoresis after site-directed mutagenesis ‘Quickchange’ protocol.
    -Wells 2 to 4: PastPfu FLY TGAP231
    -Wells 5 to 7: KD Plus TGAP301
    -Wells 8-9: Pfu Ultra (Agilent)

    Bonjour Simon,

    J’aimerais savoir le prix de ta polymerase TransStart FastPfu FLY DNA Polymerase. Je l’ai essayée et elle va très bien. Je vais essayer de convaincre mon patron pour en avoir. Je ne peux pas te dévoiler le vecteur utilisé pour cause de confidentialité. Merci!


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