DNase I (RNase-free) – GD201
DNase I (RNase-free)
Deoxyribonuclease I (DNase I) is an endonuclease that degrades double- and single-stranded DNA and chromatin. It functions by hydrolyzing phosphodiester linkages, producing mono and oligonucleotides with a 5’-phosphate and a 3’-hydroxyl group. Ribonuclease has been reduced to non-detectable levels. Its activity depends on Mg2+ or Mn2+ ion. DNase I with Mg2+ randomly cuts double stranded DNA at any sites, DNase I with Mn2+ cuts double stranded DNA at the same site to form sticky ends with 1-2 nucleotide overhangs or blunt ends. DNase I is included in most of our RNA extraction kits.
Purified from bovine pancreas.
One unit is the amount of enzyme required to completely degrade 1 μg pBR322 plasmid DNA in 10 minutes at 37°C.
- DNase I footprinting
- Nick translation
- Remove DNA from RNA preparation
at -20°C for two years
Manual and datasheet
1 500 u
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Published on Jan 30, 2017 by Simon Roy, Ph.D Detection Limit of High-Fidelity DNA Polymerases using Human gDNA for Template We previously determined the detection limit of different Taq DNA Polymerases using human genomic DNA as a template for PCR. For this instance,...
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