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DNase I (RNase-free) – GD201

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SKU: GD201Category: Tag: Brand:

DNase I (RNase-free)

Deoxyribonuclease I (DNase I) is an endonuclease that degrades double- and single-stranded DNA and chromatin. It functions by hydrolyzing phosphodiester linkages, producing mono and oligonucleotides with a 5’-phosphate and a 3’-hydroxyl group. Ribonuclease has been reduced to non-detectable levels. Its activity depends on Mg2+ or Mn2+ ion. DNase I with Mg2+ randomly cuts double stranded DNA at any sites, DNase I with Mn2+ cuts double stranded DNA at the same site to form sticky ends with 1-2 nucleotide overhangs or blunt ends. DNase I is included in most of our RNA extraction kits.


Source

Purified from bovine pancreas.


Unit Definition

One unit is the amount of enzyme required to completely degrade 1 μg pBR322 plasmid DNA in 10 minutes at 37°C.


Applications

  • DNase I footprinting
  • Nick translation
  • Remove DNA from RNA preparation

 

TGGD201 content


Storage

at -20°C for two years


Manual and datasheet

GD201 DNase I (RNase-free )

Additional information

Format

1 500 u

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