Select Page

DNase I (RNase-free) – GD201

101.00$ CAD

Clear

Cashback Reward : Earn up to 10$!


SKU: GD201 Category: Tag: Brand:

DNase I (RNase-free)

Deoxyribonuclease I (DNase I) is an endonuclease that degrades double- and single-stranded DNA and chromatin. It functions by hydrolyzing phosphodiester linkages, producing mono and oligonucleotides with a 5’-phosphate and a 3’-hydroxyl group. Ribonuclease has been reduced to non-detectable levels. Its activity depends on Mg2+ or Mn2+ ion. DNase I with Mg2+ randomly cuts double stranded DNA at any sites, DNase I with Mn2+ cuts double stranded DNA at the same site to form sticky ends with 1-2 nucleotide overhangs or blunt ends. DNase I is included in most of our RNA extraction kits.


Source

Purified from bovine pancreas.


Unit Definition

One unit is the amount of enzyme required to completely degrade 1 μg pBR322 plasmid DNA in 10 minutes at 37°C.


Applications

  • DNase I footprinting
  • Nick translation
  • Remove DNA from RNA preparation

 

TGGD201 content


Storage

at -20°C for two years


Manual and datasheet

GD201 DNase I (RNase-free )

Additional information

Format

1 500 u

Related Products

DNA Polymerase Comparison Chart

  Specifications and pricing of commercially available Taq DNA Polymerases and High-Fidelity DNA Polymerases in Canada.      

Detection Limit of High-Fidelity DNA Polymerases on gDNA

Published on Jan 30, 2017 by Simon Roy, Ph.D Detection Limit of High-Fidelity DNA Polymerases using Human gDNA for Template We previously determined the detection limit of different Taq DNA Polymerases using human genomic DNA as a template for PCR. For this instance,...

Product Reviews for DNase I (RNase-free) – GD201

Reviews

There are no reviews yet.

Be the first to review “DNase I (RNase-free) – GD201”

Your email address will not be published. Required fields are marked *

Order Today!

101.00$ CAD

Clear

Cashback Reward : Earn up to 10$!


SKU: GD201 Category: Tag: Brand: