Origin and General Characteristics
Cell Name | U-2 OS [U2OS] Cell Line |
Organism | Homo sapiens, Human |
Tissue | Bone |
Morphology | Epithelial |
Growth Properties | Adherent |
Biosafety Level | 1 |
Culture Conditions and Handling
Complete Growth Medium | McCoy’s 5A +10% FBS +1% P/S |
Subculturing | Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. |
Subcultivation Ratio | 1:2-1:4 |
Medium Renewal | Every 2 to 3 days |
Cryopreservation | Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase |
Culture Conditions | Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃ |
Additional information
Format | 2 x 1E06 cells |
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