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Jurkat Cell Line (Clone E6-1) – EP-CL-0129

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SKU: EP-CL-0129 Category: Tag: Brand:

Origin and General Characteristics

Cell NameJurkat, Clone E6-1 Cell Line
OrganismHomo sapiens, Human
TissuePeripheral blood
MorphologyLymphoblast
Growth PropertiesSuspension
DescriptionsClone E6-1 cells produce large amounts of IL-2 after stimulation with phorbol esters and either lectins or monoclonal antibodies against the T3 antigen(both types of stimulants are needed to induce IL-2 production.
Biosafety Level1

Culture Conditions and Handling

Complete Growth MediumRPMI-1640 +10% FBS +1% P/S
SubculturingCultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:2-1:4.
Subcultivation Ratio1:2-1:4
Medium RenewalEvery 2 to 3 days
CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO
Storage temperature: Liquid nitrogen vapor phase
Culture ConditionsAtmosphere: Air, 95%; CO2, 5%
Temperature: 37℃

Special Features of the Cell Line

Receptor ExpressionReceptor expression: T cell antigen receptor, expressed
Antigen ExpressionCD3; Homo sapiens, expressed
Gene ExpressionInterleukin-2(Interleukin 2, Il-2), Cd3; Homo Sapiens, Expressed
ApplicationsTransfection.

Additional information

Format

1 x 1E06 cells

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