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A549/DDP Cell Line – EP-CL-0519

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SKU: EP-CL-0519 Category: Tag: Brand:

Origin and General Characteristics

Cell NameA549/DDP Cell Line
OrganismHomo sapiens, Human
TissueLung
MorphologyEpithelial
Growth PropertiesAdherent
Biosafety Level1

Culture Conditions and Handling

Complete Growth MediumRPMI-1640 +10% FBS +1-2μg/mL DDP+1% P/S
SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Subcultivation Ratio1:2-1:4
Medium RenewalEvery 2 to 3 days
CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO
Storage temperature: Liquid nitrogen vapor phase
Culture ConditionsAtmosphere: Air, 95%; CO2, 5%
Temperature: 37℃

Additional information

Format

1 x 1E06 cells

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