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A549/DDP Cell Line – EP-CL-0519

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Origin and General Characteristics

Cell Name A549/DDP Cell Line
Organism Homo sapiens, Human
Tissue Lung
Morphology Epithelial
Growth Properties Adherent
Biosafety Level 1

Culture Conditions and Handling

Complete Growth Medium RPMI-1640 +10% FBS +1-2μg/mL DDP+1% P/S
Subculturing Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Subcultivation Ratio 1:2-1:4
Medium Renewal Every 2 to 3 days
Cryopreservation Freeze medium: 60% Basal medium+30% FBS+10% DMSO
Storage temperature: Liquid nitrogen vapor phase
Culture Conditions Atmosphere: Air, 95%; CO2, 5%
Temperature: 37℃

Additional information

Format

1 x 1E06 cells

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