SUM159PT cell line – EP-CL-0622

Name: SUM159PT cell line - EP-CL-0622
Brand: Elabscience
SKU: EP-CL-0622
Price: 772.00 CAD
Availability: BackOrder

$772.00 CAD

0 in stock (expect 7-21 days for delivery)

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SKU: EP-CL-0622 Category: Cell Lines Tag: Cell Line Brand: Elabscience

Culture Conditions and Handling

Complete Growth Medium	RPMI-1640+10% FBS+1% P/S
Subculturing	Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Subcultivation Ratio	1:2-1:5
Medium Renewal	Every 2 to 3 days
Cryopreservation	Freeze Medium: 55% Basal Medium+40% FBS+5% DMSO Storage Temperature: Liquid Nitrogen Vapor Phase
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃

Additional information

Format	1 x 1E06 cells
Supplier	Elabscience

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