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A-375 Cell Line – EP-CL-0014

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Origin and General Characteristics

Cell Name A-375 Cell Line
Organism Homo sapiens, Human
Age 54 years
Tissue Skin
Morphology Epithelial
Growth Properties Adherent
Descriptions The A-375 cell line was established by D.J. Giard in 1973.
Biosafety Level 1

Culture Conditions and Handling

Complete Growth Medium DMEM +10% FBS +1% P/S
Subculturing Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Subcultivation Ratio 1:3-1:6
Medium Renewal Every 2 to 3 days
Cryopreservation Freeze medium: 60% Basal medium+30% FBS+10% DMSO
Storage temperature: Liquid nitrogen vapor phase
Culture Conditions Atmosphere: Air, 95%; CO2, 5%
Temperature: 37℃

Special Features of the Cell Line

Tumorigenic Yes
Effects Yes, in immunosuppressed mice.
Applications Transfection host.

Additional information

Format

2 x 1E06 cells

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