A-375 Cell Line – EP-CL-0014

$449.00 CAD

SKU: N/A Category: Cell Lines Tag: Cell Line Brand: Elabscience

Cell Name	A-375 Cell Line
Organism	Homo sapiens, Human
Age	54 years
Tissue	Skin
Morphology	Epithelial
Growth Properties	Adherent
Descriptions	The A-375 cell line was established by D.J. Giard in 1973.
Biosafety Level	1

Complete Growth Medium	DMEM +10% FBS +1% P/S
Subculturing	Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Subcultivation Ratio	1:3-1:6
Medium Renewal	Every 2 to 3 days
Cryopreservation	Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions	Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Tumorigenic	Yes
Effects	Yes, in immunosuppressed mice.
Applications	Transfection host.

Additional information

Format	2 x 1E06 cells
Supplier	Elabscience

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