Origin and General Characteristics
| Cell Name | A-375 Cell Line |
| Organism | Homo sapiens, Human |
| Age | 54 years |
| Tissue | Skin |
| Morphology | Epithelial |
| Growth Properties | Adherent |
| Descriptions | The A-375 cell line was established by D.J. Giard in 1973. |
| Biosafety Level | 1 |
Culture Conditions and Handling
| Complete Growth Medium | DMEM +10% FBS +1% P/S |
| Subculturing | Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. |
| Subcultivation Ratio | 1:3-1:6 |
| Medium Renewal | Every 2 to 3 days |
| Cryopreservation | Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃ |
Special Features of the Cell Line
| Tumorigenic | Yes |
| Effects | Yes, in immunosuppressed mice. |
| Applications | Transfection host. |
Additional information
| Format | 2 x 1E06 cells |
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