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NCI-H2228 Cell Line – EP-CL-0570

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Origin and General Characteristics

Cell NameNCI-H2228 [H2228; H-2228] Cell Line
OrganismHomo sapiens, Human
AgeFemale
TissueLung
MorphologyEpithelial
Growth PropertiesAdherent
DescriptionsThe line was established in August 1989 from lung adenocarcinoma. The tissue donor was a non-smoker.
Biosafety Level1

Culture Conditions and Handling

Complete Growth MediumRPMI-1640 +10% FBS +Glutamax+1% P/S
SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Subcultivation Ratio1:2-1:4
Medium RenewalEvery 2 to 3 days
CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO
Storage temperature: Liquid nitrogen vapor phase
Culture ConditionsAtmosphere: Air, 95%; CO2, 5%
Temperature: 37℃
TumorigenicYes
EffectsYes, forms tumors in nude mice.

Special Features of the Cell Line

It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).

Additional information

Format

1 x 1E06 cells

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