NCI-H2228 Cell Line – EP-CL-0570
$693.00 CAD
0 in stock (expect 7-21 days for delivery)
Origin and General Characteristics
| Cell Name | NCI-H2228 [H2228; H-2228] Cell Line |
| Organism | Homo sapiens, Human |
| Age | Female |
| Tissue | Lung |
| Morphology | Epithelial |
| Growth Properties | Adherent |
| Descriptions | The line was established in August 1989 from lung adenocarcinoma. The tissue donor was a non-smoker. |
| Biosafety Level | 1 |
Culture Conditions and Handling
| Complete Growth Medium | RPMI-1640 +10% FBS +Glutamax+1% P/S |
| Subculturing | Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. |
| Subcultivation Ratio | 1:2-1:4 |
| Medium Renewal | Every 2 to 3 days |
| Cryopreservation | Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃ |
| Tumorigenic | Yes |
| Effects | Yes, forms tumors in nude mice. |
Special Features of the Cell Line
It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).
Additional information
| Format | 1 x 1E06 cells |
|---|---|
| Supplier |
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