22rv1 Cell line – EP-CL-0004

$449.00 CAD

SKU: N/A Category: Cell Lines Tag: Cell Line Brand: Elabscience

Cell Name	22RV1 Cell Line
Organism	Homo sapiens, Human
Tissue	Prostate
Morphology	Epithelial
Growth Properties	Adherent
Descriptions	The cell line expresses prostate specific antigen(PSA). Growth is weakly stimulated by dihydroxytestosterone and lysates are immunoreactive with androgen receptor antibody by Western blot analysis.Growth is stimulated by epidermal growth factor(EGF) but is not inhibited by transforming growth factor beta-1(TGF beta- 1).Recently , it has been shown that 22Rv1 prostate carcinoma cells produce high-titer of the human retrovirus XMRV(xenotropic murine leukemia virus-related virus).
Biosafety Level	2

Complete Growth Medium	RPMI-1640 +10% FBS +1% P/S
Subculturing	Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Subcultivation Ratio	1:3-1:6
Medium Renewal	Every 2 to 3 days
Cryopreservation	Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
Culture Conditions	Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Tumorigenic	Yes
Effects	Yes, forms tumors in nude mice.

Additional information

Format	2 x 1E06 cells
Supplier	Elabscience

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