Rat SOD4 (Superoxide Dismutase 4, Copper Chaperone) ELISA Kit
The SOD4 ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with SOD4. During the reaction, Rat SOD4 in the sample or standard competes with a fixed amount of SOD4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat SOD4. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat SOD4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.<
Detected Target name: Rat Superoxide Dismutase 4, Copper Chaperone
Abbreviation : SOD4
Format : 96 rx
Reactivity : Rat
ELISA configuration : Competitive
Sensitivity : 0.094 ng/ml
Detection Range : 0.156 – 10 ng/ml
Additional information
Format | 96 rx |
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