Rat αFP (Alpha-Fetoprotein) ELISA Kit – E-EL-R0153

Rat αFP (Alpha-Fetoprotein) ELISA Kit

The αFP ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to αFP. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for αFP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain αFP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of αFP. You can calculate the concentration of αFP in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Alpha-Fetoprotein

Abbreviation : αFP

Uniprot ID : P02773

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 0.188 ng/ml

Detection Range : 0.313 – 20 ng/ml

Manual & Datasheet

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