Rat EPO (Erythropoietin) ELISA Kit – E-EL-R0007

Rat EPO (Erythropoietin) ELISA Kit

The EPO ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to EPO. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for EPO and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain EPO, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of EPO. You can calculate the concentration of EPO in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Erythropoietin

Abbreviation : EPO

Uniprot ID : P29676

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 37.5 pg/ml

Detection Range : 62.5 – 4000 pg/ml

Manual & Datasheet

More information about this Rat EPO (Erythropoietin) ELISA Kit from Elabscience