Rat GDF11 (Growth Differentiation Factor 11) ELISA Kit
The GDF11 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GDF11. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for GDF11 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GDF11, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of GDF11. You can calculate the concentration of GDF11 in the samples by comparing the OD of the samples to the standard curve.
Detected Target name: Rat Growth Differentiation Factor 11
Abbreviation : GDF11
Uniprot ID : Q9Z217
Format : 96 rx
Reactivity : Rat
ELISA configuration : Sandwich
Sensitivity : 9.375 pg/ml
Detection Range : 15.625 – 1000 pg/ml
More information about this Rat GDF11 (Growth Differentiation Factor 11) ELISA Kit from Elabscience
Additional information
Format | 96 rx |
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