Rat DHEA-S (Dehydroepiandrosterone Sulfate) ELISA Kit – E-EL-R0325

Rat DHEA-S (Dehydroepiandrosterone Sulfate) ELISA Kit

The DHEA-S ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with DHEA-S. During the reaction, Rat DHEA-S in the sample or standard competes with a fixed amount of DHEA-S on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat DHEA-S. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat DHEA-S in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: Rat Dehydroepiandrosterone Sulfate

Abbreviation : DHEA-S

Uniprot ID :

Format : 96 rx

Reactivity : Rat

ELISA configuration : Competitive

Sensitivity : 18.75 pg/ml

Detection Range : 31.25 – 2000 pg/ml

Manual & Datasheet

More information about this Rat DHEA-S (Dehydroepiandrosterone Sulfate) ELISA Kit from Elabscience