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Rat ECF/CCL11 (Eosinophil Chemotactic Factor) ELISA Kit – E-EL-R0368

96 rx

$869.00 CAD

0 in stock (expect 7-21 days for delivery)

0 in stock (expect 7-21 days for delivery)

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SKU: E-EL-R0368 Category: Tags: , Brand:

Rat ECF/CCL11 (Eosinophil Chemotactic Factor) ELISA Kit

The ECF/CCL11 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ECF/Eotaxin 1/CCL11. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for ECF/Eotaxin 1/CCL11 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ECF/Eotaxin 1/CCL11, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of ECF/Eotaxin 1/CCL11. You can calculate the concentration of ECF/Eotaxin 1/CCL11 in the samples by comparing the OD of the samples to the standard curve.


Detected Target name: Rat Eosinophil Chemotactic Factor


Abbreviation : ECF/CCL11


Uniprot ID : P97545


Format : 96 rx


Reactivity : Rat


ELISA configuration : Sandwich


Sensitivity : 18.75 pg/ml


Detection Range : 31.25 – 2000 pg/ml


Manual & Datasheet


More information about this Rat ECF/CCL11 (Eosinophil Chemotactic Factor) ELISA Kit from Elabscience


Additional information

Format

96 rx

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