Rat ECF/CCL11 (Eosinophil Chemotactic Factor) ELISA Kit
The ECF/CCL11 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ECF/Eotaxin 1/CCL11. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for ECF/Eotaxin 1/CCL11 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ECF/Eotaxin 1/CCL11, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of ECF/Eotaxin 1/CCL11. You can calculate the concentration of ECF/Eotaxin 1/CCL11 in the samples by comparing the OD of the samples to the standard curve.
Detected Target name: Rat Eosinophil Chemotactic Factor
Abbreviation : ECF/CCL11
Uniprot ID : P97545
Format : 96 rx
Reactivity : Rat
ELISA configuration : Sandwich
Sensitivity : 18.75 pg/ml
Detection Range : 31.25 – 2000 pg/ml
More information about this Rat ECF/CCL11 (Eosinophil Chemotactic Factor) ELISA Kit from Elabscience
Additional information
Format | 96 rx |
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