Rat ALPI (Alkaline Phosphatase, Intestinal) ELISA Kit – E-EL-R0139

Rat ALPI (Alkaline Phosphatase, Intestinal) ELISA Kit

The ALPI ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ALPI. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for ALPI and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ALPI, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of ALPI. You can calculate the concentration of ALPI in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Alkaline Phosphatase, Intestinal

Abbreviation : ALPI

Uniprot ID : P15693

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 0.469 ng/ml

Detection Range : 0.781 – 50 ng/ml

Manual & Datasheet

More information about this Rat ALPI (Alkaline Phosphatase, Intestinal) ELISA Kit from Elabscience