Rat GTF IIB (General Transcription Factor IIB) ELISA Kit – E-EL-R0417

Rat GTF IIB (General Transcription Factor IIB) ELISA Kit

The GTF IIB ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GTF IIB. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat GTF IIB and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GTF IIB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat GTF IIB. You can calculate the concentration of Rat GTF IIB in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat General Transcription Factor IIB

Abbreviation : GTF IIB

Uniprot ID : P62916

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 37.5 pg/ml

Detection Range : 62.5 – 4000 pg/ml

Manual & Datasheet

More information about this Rat GTF IIB (General Transcription Factor IIB) ELISA Kit from Elabscience