Rat CTXIII ( Cross-linked C-Telopeptides Of Type III Collagen) ELISA Kit – E-EL-R0281

Rat CTXIII ( Cross-linked C-Telopeptides Of Type III Collagen) ELISA Kit

The CTXIII ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CTXIII. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for CTXIII and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CTXIII, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of CTXIII. You can calculate the concentration of CTXIII in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Cross-linked C-Telopeptides Of Type III Collagen

Abbreviation : CTXIII

Uniprot ID :

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 0.094 ng/ml

Detection Range : 0.156 – 10 ng/ml

Manual & Datasheet

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