Rat CDK5 ( Cyclin Dependent Kinase 5) ELISA Kit – E-EL-R0291

Rat CDK5 ( Cyclin Dependent Kinase 5) ELISA Kit

The CDK5 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CDK5. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat CDK5 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CDK5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CDK5. You can calculate the concentration of Rat CDK5 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Cyclin Dependent Kinase 5

Abbreviation : CDK5

Uniprot ID : Q03114

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 0.188 ng/ml

Detection Range : 0.313 – 20 ng/ml

Manual & Datasheet

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