Rat ADORA3 (Adenosine A3 Receptor) ELISA Kit – E-EL-R2510

Rat ADORA3 (Adenosine A3 Receptor) ELISA Kit

The ADORA3 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ADORA3. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat ADORA3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ADORA3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ADORA3. You can calculate the concentration of Rat ADORA3 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Adenosine A3 Receptor

Abbreviation : ADORA3

Uniprot ID : P28647

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 0.188 ng/ml

Detection Range : 0.313 – 20 ng/ml

Manual & Datasheet

More information about this Rat ADORA3 (Adenosine A3 Receptor) ELISA Kit from Elabscience