Rat GLUT4 (Glucose Transporter 4) ELISA Kit – E-EL-R0430

Rat GLUT4 (Glucose Transporter 4) ELISA Kit

The GLUT4 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Glut4. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Glut4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Glut4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Glut4. You can calculate the concentration of Glut4 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Glucose Transporter 4

Abbreviation : GLUT4

Uniprot ID : P19357

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 0.375 ng/ml

Detection Range : 0.625 – 40 ng/ml

Manual & Datasheet

More information about this Rat GLUT4 (Glucose Transporter 4) ELISA Kit from Elabscience