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PerfectStart Taq DNA Polymerase (no E.Coli gDNA) – AP401

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PerfectStart® Taq DNA Polymerase

PerfectStart Taq DNA Polymerase is a hot start Taq DNA polymerase containing Taq DNA polymerase and three unique monoclonal antibodies that effectively block DNA polymerase activity and prevent non-specific amplification. As the denaturation step progresses, the antibodies are inactivated and PerfectStart Taq is activated, The amplified product posess a 3′ A overhang due to terminal transferase activity of Taq DNA Polymerase. Such DNA ends are suitable for T/A cloning in pEASY vectors..

As compared to TransStart Taq DNA Polymerase AP141 and TransSrart® TopTaq DNA Polymerase AP151, PerfectStart Taq DNA Polymerase AP401 shows higher amplification efficiency, specificity and sensitivity.

  • The reagents provided with AP401 have been optimized for molecular diagnosis.
  • Can be used for PCR amplification of 1 kb or less.

Advantages of PerfectStart Taq DNA Polymerase

  • Molecular diagnosis -grade purity and quality
  • Less than 1 copy of residual gDNA from E.Coli
  • PCR setup can be performed at room temperature
  • Benefit from reduced nonspecific amplification,
  • Minimized primer dimer fomlation.
  • PCR products can be directly cloned into pEASY®-T topo cloning vectors
  • Available with or without 2.5 mM dNTPs for additional convenience.

AP401, PerfectStart Taq DNA Polymerase, can be used for PCR including

  • Low copy number templates.
  • Complex templates.
  • GC/AT-rich templates.
  • Real-time Quantitative PCR (qPCR).
  • Multiplex PCR.
  • Single nucleotide polymorphisms (SNPs) detection.

AP401 has passed the following quality control assays

  • functional absence of double-and single-stranded endonuclease activity;
  • >99% homogeneousness measured by SDS-PAGE,
  • host-free residual DNA detected by PCR method,
  • effective amplification of single-copy genes from human genome.

Kit Contents, Manual and datasheet

AP401 – PerfectStart Taq DNA Polymerase

Additional information

Format

6 x 500u, 6 x 500u + dNTPs, 250u, 250u + dNTPs, 500u, 500u + dNTPs

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