PerfectStart Taq DNA Polymerase (no E.Coli gDNA) – AP401

PerfectStart® Taq DNA Polymerase

PerfectStart Taq DNA Polymerase is a hot start Taq DNA polymerase containing Taq DNA polymerase and three unique monoclonal antibodies that effectively block DNA polymerase activity and prevent non-specific amplification. As the denaturation step progresses, the antibodies are inactivated and PerfectStart Taq is activated, The amplified product posess a 3′ A overhang due to terminal transferase activity of Taq DNA Polymerase. Such DNA ends are suitable for T/A cloning in pEASY vectors..

As compared to TransStart Taq DNA Polymerase AP141 and TransSrart® TopTaq DNA Polymerase AP151, PerfectStart Taq DNA Polymerase AP401 shows higher amplification efficiency, specificity and sensitivity.

• The reagents provided with AP401 have been optimized for molecular diagnosis.
• Can be used for PCR amplification of 1 kb or less.

Advantages of PerfectStart Taq DNA Polymerase

• Molecular diagnosis -grade purity and quality
• Less than 1 copy of residual gDNA from E.Coli
• PCR setup can be performed at room temperature
• Benefit from reduced nonspecific amplification,
• Minimized primer dimer fomlation.
• PCR products can be directly cloned into pEASY®-T topo cloning vectors
• Available with or without 2.5 mM dNTPs for additional convenience.

AP401, PerfectStart Taq DNA Polymerase, can be used for PCR including

• Low copy number templates.
• Complex templates.
• GC/AT-rich templates.
• Real-time Quantitative PCR (qPCR).
• Multiplex PCR.
• Single nucleotide polymorphisms (SNPs) detection.

AP401 has passed the following quality control assays

• functional absence of double-and single-stranded endonuclease activity;
• >99% homogeneousness measured by SDS-PAGE,
• host-free residual DNA detected by PCR method,
• effective amplification of single-copy genes from human genome.

Kit Contents, Manual and datasheet

AP401 – PerfectStart Taq DNA Polymerase