2x TransTaq®-T PCR SuperMix (-dye) – AS122

2x TransTaq®-T PCR SuperMix (-dye)

TransTaq-T PCR SuperMix is a ready-to-use mixture of TransTaq-T DNA Polymerase, dNTPs and optimized buffer. The supermix is provided at 2x concentration and used at 1x concentration by adding template, primers and H₂O. Efficiency of PCR products with “A” is equal to EasyTaq^® DNA polymerase. It is however more suitable for high-fidelity TA cloning than EasyTaq DNA polymerase.

Advantages of using 2x TransTaq-T PCR SuperMix

• Fidelity is 18 times higher than EasyTaq^® DNA Polymerase.
• The extension rate is about 1-2 kb/min.
• Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be cloned into pEASY^®-T vectors.
• Amplification of genomic DNA fragment up to 8 kb.

Manual and datasheet

AS122 – TransTaq-T PCR Supermix