TransScript® One-Step RT-PCR SuperMix (+dye) – AT411

TransScript® One-Step RT-PCR SuperMix (+dye)

TransScript® One-Step RT-PCR SuperMix combines the first-strand cDNA synthesis with PCR in the same tube to simplify reaction setup and reduce the possibility of contamination. Only gene-specific primers can be used for One-Step RT-PCR. TransScript RT and TransTaq HiFi DNA Polymerase are used in the kit.

Advantages

• High efficiency cDNA synthesis with TransScript One-Step Enzyme Mix and 2×One-Step Reaction Mix, followed by PCR amplification with resulting cDNA. Simple procedure helps to minimize contamination.
• Amplification product contains base “A” at 3’ end. Purified product is well suitable for cloning into pEASY-T vectors.
• Amplified fragment up to 8 kb.
• RNase inhibitor included in the mix.

Manual and Datasheet

AT411 TransScript® One-Step RT-PCR SuperMix