TransScript® II All-in-One First-Strand cDNA Synthesis SuperMix for PCR – AH321

TS II All-in-One First-Strand cDNA Synthesis SuperMix for PCR

TransScript II All-in-One First-Strand cDNA Synthesis SuperMix for PCR provides all the necessary components for cDNA synthesis from total RNA or mRNA. The supermix is provided at 5x concentration and used at 1x concentration by adding RNA and H₂O. The resulting cDNA are suitable for regular PCR but not for qPCR.

Advantages of the TransScript II All-in-One First-Strand cDNA Synthesis SuperMix

• One-tube format for simple and fast setup by adding RNA template and water.
• The optimal ratio of oligo(dT)20 primer to random primer(N9) and optimized SuperMix components allow high efficiency synthesis of long cDNA.
• Reverse transcription in only 30 minutes
• High compatibility with PCR reagents
• cDNA fragment up to 15 kb
• RNase inhibitor included in the mix.

Manual and Datasheet

AH321 TransScript® II All-in-One First-Strand cDNA Synthesis SuperMix for PCR