2x TransTaq® High Fidelity (HiFi) PCR SuperMix I (-dye)

TransTaq^® High Fidelity (HiFi) PCR SuperMix I or II is a ready-to-use mixture of TransTaq^® High Fidelity (HiFi) DNA polymerase, with a proofreading 3’-5’ exonuclease, dNTPs and optimized buffer. TransTaq^® High Fidelity (HiFi) PCR SuperMix I is optimized for the amplification of genomic DNA and PCR SuperMix II is optimized for the amplification of λDNA, cDNA or plasmid DNA. The supermix is provided at 2x concentration and can be used at 1x concentration by adding template, primers and H2O.

Advantages

• Optimized buffer for genomic DNA templates (i.e genotyping)
• Fidelity is 18 times higher than EasyTaq^® DNA Polymerase.
• The extension rate is about 1-2 kb/min.
• Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned into pEASY®-Tvectors.
• Amplification of genomic DNA fragment up to 15 kb.

Manual and datasheet:

2x TransTaqTM High Fidelity (HiFi) PCR SuperMix I、II