pEASY®-T3 Cloning Kit – CT301

pEASY®-T3 T/A TOPO Cloning Kit

pEASY®-T3 Cloning Vector provides dual-Eco RI and dual-Not I restriction sites. It is designed for cloning and sequencing Taq-amplified PCR products 3’A-overhangs). The cloned insert can be released from a single enzyme digestion. The vector is suitable for T7 RNA polymerase-mediated In vitro transcription using our T7 High-Efficiency Transcription Kit. Visit our DNA Polymerase selection chart to find the best DNA polymerase suitable for T/A cloning.

Features of the pEASY®-T3 Cloning Kit

• 5 minutes fast ligation of Taq-amplified (‘A overhangs) PCR products.
• Ampicillin resistance genes for your choice of selection.
• Easy blue/white selection.
• T7 promoter, SP6 promoter, M13 forward and M13 reverse primers for sequencing.
• T7 promoter for in vitro transcription.
• Includes Trans1-T1 phage resistant chemically competent cell, high transformation efficiency (>10⁹ cfu/μg pUC19 DNA) and fast growth.

Datasheet and manual

CT301 pEASY®-T3 Cloning Kit

CT301 pEASY®-T3 vector Snapgene

T3 complete sequence