DNA Polymerase Selection Chart

Which DNA Polymerase should I use ?

DNA Polymerase Selection can be difficult to make. Choosing the most suitable DNA polymerase for amplifying DNA in PCR reactions according to your specific needs can be challenging. DNA Polymerase Selection is crucial. Choosing from Genuine Taq (not blended), Enhanced Taq (Taq + Pfu blends), High-Fidelity to Ultra-High-Fidelity (Pfu of KOD) DNA Polymerases highly depends on your specific application and needs. DNA Polymerase Selection for PCR application highly depends on the characteristics of the system as well as the desired results. For instance, genotyping, cloning, library amplification and site-directed mutagenesis require different DNA polymerases. In addition, GC-rich, AT-rich and high complexity DNA templates may require a more robust DNA Polymerase. Civic Bioscience has built a DNA Polymerase Selection chart of industry-leading DNA polymerases that can help you find the best DNA polymerase for your needs.

PCR: the Price of Infidelity (Nature)Download and Print the Chart

DNA Polymerase Selection Chart with all specifications

DNA Polymerase	HotStart Type	Sub-Category	Fidelity	3' end	Extension Rate	Amplicon size (gDNA/plasmid)	GC-rich /complex	Efficiency + Specificity	Cat. No	Max Price (CAD) per 50 ul rx
TransFast® Taq DNA Polymerase (6 kb/min)	None	Genuine Taq	1x	A	6 kb/min	4 kb	+	+	AP101	0,29 $
EasyTaq® DNA Polymerase	None	Genuine Taq	1x	A	1-2 kb/min	4 kb	+	+	AP111	0,26 $
TransTaq®-T DNA Polymerase	Single	Enhanced Taq+Pfu	18x	A	1-2 kb/min	8 kb	++	++	AP121	0,51 $
TransTaq® (HiFi) DNA Polymerase High-Fidelity	Single	Enhanced Taq+Pfu	18x	A	1-2 kb/min	15 kb	+++	+++	AP131	0,75 $
TransStart® Taq DNA Polymerase	Double	Genuine Taq	18x	A	1-2 kb/min	15 kb	+	++++	AP141	0,48 $
TransStart® TopTaq DNA Polymerase	Double	Genuine Taq	18x	A	1-2 kb/min	15 kb	++	+++++	AP151	0,57 $
EasyPfu DNA Polymerase	None	High-Fidelity	18x	Blunt	0,5 kb/min	6 kb / 10 kb	++	++	AP211	0,56 $
TransStart® FastPfu (High-Fidelity) DNA Polymerase	Double	High-Fidelity	54x	Blunt	2-4 kb/min	15 kb / 20 kb	+++	++++	AP221	1,50 $
TransStart® FastPfu FLY (Ultra-HiFi) DNA Polymerase	Double	Ultra-High Fidelity	108x	Blunt	2-6 kb/min	15 kb / 20 kb	+++	+++++	AP231	1,73 $
TransStart® KD Plus (Ultra-HiFi) DNA Polymerase	Double	Ultra-High Fidelity	108x	Blunt	1 kb/min	15 kb / 20 kb	+++	++++	AP301	1,24 $

DNA Polymerase Selection Chart - Updated on 2017-01-08
Max price per 50 ul reaction is based on the smallest pack size (-01) without dNTPs or other reagent and plastic consumable.

DNA Polymerase Selection: Application Chart

At room temperature, one binding protein binds to double-strand DNA template and another binding protein binds to primer. These unique formulations effectively neutralize the DNA polymerase activity at room temperature. Blocking proteins are released from templates and primers during the initial denaturation. This double blocking method has higher efficiency than antibody based, or chemically modified hot start PCR.

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