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pEASY-Basic Seamless Cloning and DNA Assembly Kit – CU201

Custom kit available for only 272 $ when combined with any High-Fidelity DNA Polymerase

$189.00$489.00 CAD

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Basic Seamless Cloning and DNA Assembly

The pEASY-Basic Seamless Cloning and Assembly Kit takes advantage of proprietary recombinases and homologous recombination to achieve directional recombination of PCR inserts with 15-25bp overlapping ends into any linearized vector, enabling efficient and seamless assembly of 1-5 fragments.


Features of the pEASY-Basic CU201

  • Fast assembly and fast cloning: 15 minutes
  • Easy: regardless of restriction enzyme sites of inserts, and elimination of restriction digestion of inserts
  • High efficiency: up to 95% positive clones
  • Seamless Assembly: no extra sequences introduced
  • Includes High-Efficiency Trans1-T1 Phage Chemically Competent Cells.

 

Component CU201-02 CU201-03
2×Basic Assembly Mix 100 μl 6×100 μl
Trans1-T1 Phage Resistant Chemically Competent Cell 10×100 μl 30×100 μl
Linearized pUC19 Control Vector (10 ng/μl) 6 μl 18 μl
Control Insert (1 kb,20 ng/μl) 6 μl 18 μl

 

 


TGCU101 principle


Datasheet and manual

CU201 pEASY-Basic Seamless Cloning and Assembly Kit

Additional information

Format

20 rx, 60 rx

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