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FlyCut™ Not I – JN401

(1 customer review)

$57.00$111.00 CAD

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FlyCut™ Not I

FlyCut™ Not I is expressed and purified from E.coli that carries the recombinant NotI gene. The molecular weight is 43.3 kDa, with the recognition site at GC^GGCCGC. The reaction condition is at 37 °C, with heat inactivation at 65 °C for 20 minutes. This enzyme is not sensitive to dam or dcm methylation, but sensitive to mammalian CpG methylation.

Not I Enzyme Properties

Fast cleavage in 5-10 minutes with high fidelity

FlyCut™ NotI components

  • FlyCut™ Not I : 250 u / 500 u
  • 10x FlyCut™ Buffer: 1 ml / 1 ml
  • 10x DNA Loading Buffer: 1 ml / 1 ml

Manual & Datasheet

JN401 – FlyCut™ Not I

Additional information


250 u, 500 u


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Reviews for FlyCut™ Not I – JN401

1 review for FlyCut™ Not I - JN401

  1. Phil Roche

    Reliable restriction enzyme, and sames as others we’ve purchased from Civic; worked rapidly and to product specification

    • Simon Roy

      Thank you again for your feedback for NotI from Transgen Biotech restriction enzyme

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