pEASY-Uni Seamless Cloning and DNA Assembly Kit – CU101
220.00$ – 951.00$CAD
Seamless Cloning and DNA Assembly
The pEASY-Uni Seamless Cloning and Assembly Kit takes advantage of a proprietary assembly DNA assembly mix to achieve directional cloning of PCR fragments that share 15-25 bp overlapping sequences into any linearized vector. Similarly to Gibson Assembly ® and other DNA assembly enzyme mixes, this kit is highly efficient for seamless assembly of up to 5 DNA fragments.
Features of the pEASY-Uni Seamless Cloning and Assembly Kit
- Fast assembly and fast cloning: 15 minutes
- Easy: regardless of restriction enzyme sites of inserts, and elimination of restriction digestion of inserts
- High efficiency: up to 95% positive clones
- Seamless Assembly: no extra sequences introduced
- Includes High-EfficiencyTrans1-T1 Phage Chemically Competent Cells.
Note from CivicBio
We use this product to assemble 2 to 5 DNA fragments on a daily basis. Our overall success rate over 2 years (more than 200 different DNA assemblies) is about 98% and for most plasmids, over 90% of colonies will be correct. But in some circumstances, it may drop to 5-10% only. In our hands, such low success rate was caused by the actual genes/DNA sequences we were cloning. Difficult genes include genes coding for helicases, recombinases, polymerases and other genes related to the replication or transcription of genomic DNA in humans.
| Component | CU101-11 | CU101-13 |
| 2×Assembly Mix | 100 μl | 6×100 μl |
| Trans1-T1 Phage Resistant Chemically Competent Cell | 5×100 μl | 30×100 μl |
| Linearized pUC19 Control Vector (10 ng/μl) | 3 μl | 18 μl |
| Control Insert (1 kb，20 ng/μl） | 3 μl | 18 μl |
Datasheet and manual
Additional information
| Format | 1 x 100 ul, 2 x 100 ul, 6 x 100 ul |
|---|---|
| Supplier |
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CUSTOM KIT – High-Fidelity DNA Polymerase + Cloning Kit + Competent Cells
From: 114.00$CAD
Christian Le Gouill (verified owner)–
We compared side by side this kit vs the Gibson cloning kit and NEBuilder HiFi DNA Assembly kit. There were no differences in the number of colonies and the % of positive clones. When your vector is well digested and the overlaps well balanced in G+C, you will get close to 100% of positive clones. For our tests, we used an assembly of 3 fragments (1 vector and 2 overlapping PCR fragments) and we obtained 90% of positive clones. If you already using other seamless assembly kits, I suggest that you try this one. It is less expensive and you also get a cashback on your orders.
Christian
Simon Roy (verified owner)–
Thanks Christian. I use the thus kit for all our cloning and mutagenesis projects. In our last project (assembly of different inserts into a vector), 11/12 colony PCRs were good (1/12 was WT) and an amazing 30 out of 30 miniprepped colonies were good! (15 different clones x 2 preps)
Hey, you deserve this :
CASHBACK x 2 on your next order!
Simon
Simon