pEASY-Uni Seamless Cloning and DNA Assembly Kit – CU101
220.00$ – 951.00$CAD
Seamless Cloning and DNA Assembly
The pEASY-Uni Seamless Cloning and Assembly Kit takes advantage of a proprietary assembly DNA assembly mix to achieve directional cloning of PCR fragments that share 15-25 bp overlapping sequences into any linearized vector. Similarly to Gibson Assembly ® and other DNA assembly enzyme mixes, this kit is highly efficient for seamless assembly of up to 5 DNA fragments.
Features of the pEASY-Uni Seamless Cloning and Assembly Kit
- Fast assembly and fast cloning: 15 minutes
- Easy: regardless of restriction enzyme sites of inserts, and elimination of restriction digestion of inserts
- High efficiency: up to 95% positive clones
- Seamless Assembly: no extra sequences introduced
- Includes High-EfficiencyTrans1-T1 Phage Chemically Competent Cells.
Note from CivicBio
We use this product to assemble 2 to 5 DNA fragments on a daily basis. Our overall success rate over 2 years (more than 200 different DNA assemblies) is about 98% and for most plasmids, over 90% of colonies will be correct. But in some circumstances, it may drop to 5-10% only. In our hands, such low success rate was caused by the actual genes/DNA sequences we were cloning. Difficult genes include genes coding for helicases, recombinases, polymerases and other genes related to the replication or transcription of genomic DNA in humans.
|2×Assembly Mix||100 μl||6×100 μl|
|Trans1-T1 Phage Resistant Chemically Competent Cell||5×100 μl||30×100 μl|
|Linearized pUC19 Control Vector (10 ng/μl)||3 μl||18 μl|
|Control Insert (1 kb，20 ng/μl）||3 μl||18 μl|
Datasheet and manual
1 x 100 ul, 2 x 100 ul, 6 x 100 ul