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pEASY-Uni Seamless Cloning and DNA Assembly Kit – CU101

Assemble up to 7 DNA fragments

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(1 customer review)

$240.00 – $1,037.00 CAD

Cashback Reward : Earn up to 51$!

SKU: CU101 Categories: , Tags: , , ,

pEASY-Uni Seamless Cloning and DNA Assembly

The pEASY-Uni Seamless Cloning and Assembly Kit takes advantage of a proprietary assembly DNA assembly mix to achieve directional cloning of PCR fragments that share 15-25 bp overlapping sequences into any linearized vector. Similarly to Gibson Assembly ® and other DNA assembly enzyme mixes, this kit is highly efficient for seamless assembly of up to 7 DNA fragments.

Features of the pEASY-Uni Seamless Cloning and Assembly Kit

  • Fast assembly and fast cloning: 15 minutes
  • Easy: regardless of restriction enzyme sites of inserts, and elimination of restriction digestion of inserts
  • High efficiency: up to 95% positive clones
  • Seamless Assembly: no extra sequences introduced
  • Includes High-Efficiency Trans1-T1 Phage Chemically Competent Cells.

 

Note from CivicBio

We use this product to assemble 2 to 5 DNA fragments on a daily basis. Our overall success rate over 2 years (more than 200 different DNA assemblies) is about 98% and for most plasmids, over 90% of colonies will be correct. But in some circumstances, it may drop to 5-10% only. In our hands, such low success rate was caused by the actual genes/DNA sequences we were cloning. Difficult genes include genes coding for helicases, recombinases, polymerases and other genes related to the replication or transcription of genomic DNA in humans.

 

Component CU101-11 CU101-13
2×Assembly Mix 100 μl 6×100 μl
Trans1-T1 Phage Resistant Chemically Competent Cell 5×100 μl 30×100 μl
Linearized pUC19 Control Vector (10 ng/μl) 3 μl 18 μl
Control Insert (1 kb，20 ng/μl） 3 μl 18 μl

 

 

TGCU101 principle

Datasheet and manual

CU101 pEASY-Uni Seamless Cloning and Assembly Kit

Additional information

Format

1 x 100 ul, 2 x 100 ul, 6 x 100 ul
Supplier

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Reviews for pEASY-Uni Seamless Cloning and DNA Assembly Kit – CU101

1 review for pEASY-Uni Seamless Cloning and DNA Assembly Kit - CU101

  1. Christian Le Gouill (verified owner) –

    We compared side by side this kit vs the Gibson cloning kit and NEBuilder HiFi DNA Assembly kit. There were no differences in the number of colonies and the % of positive clones. When your vector is well digested and the overlaps well balanced in G+C, you will get close to 100% of positive clones. For our tests, we used an assembly of 3 fragments (1 vector and 2 overlapping PCR fragments) and we obtained 90% of positive clones. If you already using other seamless assembly kits, I suggest that you try this one. It is less expensive and you also get a cashback on your orders.

    Christian

    • Simon Roy (verified owner) –

      Thanks Christian. I use the thus kit for all our cloning and mutagenesis projects. In our last project (assembly of different inserts into a vector), 11/12 colony PCRs were good (1/12 was WT) and an amazing 30 out of 30 miniprepped colonies were good! (15 different clones x 2 preps)

      Hey, you deserve this :

      CASHBACK x 2 on your next order!

      Simon

      Simon

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