pEASY-Uni Seamless Cloning and DNA Assembly Kit – CU101
220.00$ – 951.00$ CAD
Seamless Cloning and DNA Assembly
The pEASY-Uni Seamless Cloning and Assembly Kit takes advantage of a proprietary assembly DNA assembly mix to achieve directional cloning of PCR fragments that share 15-25 bp overlapping sequences into any linearized vector. Similarly to Gibson Assembly ® and other DNA assembly enzyme mixes, this kit is highly efficient for seamless assembly of up to 5 DNA fragments.
Features of the pEASY-Uni Seamless Cloning and Assembly Kit
- Fast assembly and fast cloning: 15 minutes
- Easy: regardless of restriction enzyme sites of inserts, and elimination of restriction digestion of inserts
- High efficiency: up to 95% positive clones
- Seamless Assembly: no extra sequences introduced
- Includes High-Efficiency Trans1-T1 Phage Chemically Competent Cells.
Note from CivicBio
We use this product to assemble 2 to 5 DNA fragments on a daily basis. Our overall success rate over 2 years (more than 200 different DNA assemblies) is about 98% and for most plasmids, over 90% of colonies will be correct. But in some circumstances, it may drop to 5-10% only. In our hands, such low success rate was caused by the actual genes/DNA sequences we were cloning. Difficult genes include genes coding for helicases, recombinases, polymerases and other genes related to the replication or transcription of genomic DNA in humans.
| Component | CU101-11 | CU101-13 |
| 2×Assembly Mix | 100 μl | 6×100 μl |
| Trans1-T1 Phage Resistant Chemically Competent Cell | 5×100 μl | 30×100 μl |
| Linearized pUC19 Control Vector (10 ng/μl) | 3 μl | 18 μl |
| Control Insert (1 kb，20 ng/μl） | 3 μl | 18 μl |
Datasheet and manual
Additional information
| Format | 1 x 100 ul, 2 x 100 ul, 6 x 100 ul |
|---|---|
| Supplier |
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Christian Le Gouill (verified owner) –
We compared side by side this kit vs the Gibson cloning kit and NEBuilder HiFi DNA Assembly kit. There were no differences in the number of colonies and the % of positive clones. When your vector is well digested and the overlaps well balanced in G+C, you will get close to 100% of positive clones. For our tests, we used an assembly of 3 fragments (1 vector and 2 overlapping PCR fragments) and we obtained 90% of positive clones. If you already using other seamless assembly kits, I suggest that you try this one. It is less expensive and you also get a cashback on your orders.
Christian
Simon Roy (verified owner) –
Thanks Christian. I use the thus kit for all our cloning and mutagenesis projects. In our last project (assembly of different inserts into a vector), 11/12 colony PCRs were good (1/12 was WT) and an amazing 30 out of 30 miniprepped colonies were good! (15 different clones x 2 preps)
Hey, you deserve this :
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Simon
Simon