pEASY-Uni Seamless Cloning and DNA Assembly Kit – CU101
220.00$ – 951.00$ CAD
Seamless Cloning and DNA Assembly
The pEASY-Uni Seamless Cloning and Assembly Kit takes advantage of a proprietary assembly DNA assembly mix to achieve directional cloning of PCR fragments that share 15-25 bp overlapping sequences into any linearized vector. Similarly to Gibson Assembly ® and other DNA assembly enzyme mixes, this kit is highly efficient for seamless assembly of up to 5 DNA fragments.
Features of the pEASY-Uni Seamless Cloning and Assembly Kit
- Fast assembly and fast cloning: 15 minutes
- Easy: regardless of restriction enzyme sites of inserts, and elimination of restriction digestion of inserts
- High efficiency: up to 95% positive clones
- Seamless Assembly: no extra sequences introduced
- Includes High-Efficiency Trans1-T1 Phage Chemically Competent Cells.
Note from CivicBio
We use this product to assemble 2 to 5 DNA fragments on a daily basis. Our overall success rate over 2 years (more than 200 different DNA assemblies) is about 98% and for most plasmids, over 90% of colonies will be correct. But in some circumstances, it may drop to 5-10% only. In our hands, such low success rate was caused by the actual genes/DNA sequences we were cloning. Difficult genes include genes coding for helicases, recombinases, polymerases and other genes related to the replication or transcription of genomic DNA in humans.
|2×Assembly Mix||100 μl||6×100 μl|
|Trans1-T1 Phage Resistant Chemically Competent Cell||5×100 μl||30×100 μl|
|Linearized pUC19 Control Vector (10 ng/μl)||3 μl||18 μl|
|Control Insert (1 kb，20 ng/μl）||3 μl||18 μl|
Datasheet and manual
1 x 100 ul, 2 x 100 ul, 6 x 100 ul
Ask a question about pEASY-Uni Seamless Cloning and DNA Assembly Kit – CU101
Product Reviews for pEASY-Uni Seamless Cloning and DNA Assembly Kit – CU101
More to Read
by Simon Roy | Sep 16th, 2019 | Latest News, Promotions | Promo code : civicbio4-50 1 per customer. Using this coupon will reduce Cashback Reward earning rate by 50%. Until Nov, 30th, 2019 Double your Cashback Rewards with any product purchase! + Cashback x1.5...
Which is the Best Kit for DNA Clean Up? Our aim was to perform a PCR Purification Kit Comparison from different vendors. At Civic Bioscience, we distribute the Favorprep™ and EasyPure® product lines from FAVORGEN and TransBionovo respectively. First, the FavorPrep™...
By purchasing any of TransBionovo's DNA Polymerase, you instantly benefit from our PCR Success Guarantee !
The Problem with Antibodies used for Research Antibodies used for Research in academic, industrial and therapeutic fields are universal reagents in molecular biology assays. Without them, the entire field of medical research could collapse. However, there are many...
GC-rich PCR: FastPfu FLY vs Q5® High-Fidelity DNA Polymerase GC-rich PCR can be difficult to achieve Our aim was to verify compare FastPfu FLY (TransBionovo) and Q5 (NEB) high-fidelity DNA Polymerase at amplifying a GC-rich sequence (human ARX; 79% GC) from purified...
What's the Detection Limit of Taq ? How many DNA copies from gDNA? There are MANY Taq DNA Polymerases out there on the market. Some are driven by strong marketing and others by strong performance. Our aim was to verify the Detection Limit of Taq DNA Polymerases in PCR...
Experimental dates range : 2018-05 to 2018-10 Published : September 22nd, 2019 by Simon Roy, Ph.D Extremely variable GC composition found in a 2.1 kb genomic DNA sequence coding for exon 1, an intron and exon 2 of the human TWIST1 gene DNA...
A huge THANK YOU for your support! MERCI encore INFINIMENT de supporter notre jeune compagnie! by Simon Roy | September 16th, 2019 | Company Posts, Latest NewsPrices for services vary case by case. 4rth anniversary promotions are coming soon! Let's CELEBRATE...
DNA Polymerases | EasyTaq | FastPfu | PCR | PCR Purification | Taq DNA PolymeraseSave on the Products that MatterOur already popular 222$ DUO Miniprep + Gel/PCR Purification now allows you to choose 2 x 300rx minipreps or 2x 300rx Gel/PCR purification. Already...