14-3-3η (14-3-3 eta) Antibody – Clone 3G12

Encor 14-3-3η (14-3-3 eta) Antibody – MCA-3G12

The 14.3.3 family of proteins was originally discovered as prominent protein spots on 2-dimensional gels. They are a family of 28-33 kDa proteins which make up the major portion of cytoplasm proteins (1). They act as binding partners for phosphoserine and phosphothreonine sites in other proteins, though they also have binding partners which are not phosphorylation-dependent. These binding interactions are important in the regulation of molecules such as signaling kinases in the MAP kinase pathway, c-Raf and b-Raf, the proapoptotic molecules Bad and Ba,x and the cell cycle regulator Cdc25. There are 7 mammalian 14.3.3 proteins and they are normally expressed as homo- or in some cases hetero-dimers. 14.3.3η or 14.3.3 eta is widely expressed and concentrated in the nervous system. The HGNC name for 14.3.3 is YWHAH, and an alternate name is tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein 1, due to this protein’s role in activation of tyrosine and tryptophan hydroxylases. The 14-3-3η protein accumulates in the CSF of patients suffering from Creutzfeld-Jacob Disease, and thus can be used for the diagnosis of this disease (2). Furthermore, this protein binds α-synuclein in the Lewy bodies of Parkinson’s disease-affected brains and has been linked to early-onset schizophrenia (3,4).

HGNC name(s) : YWHAH

Host : Mouse

Clonality : Monoclonal

Reactivity : Human | Cow | Pig | Chicken | Rat | Mouse

Isotype : IgG1

Immunogen : Recombinant full length human

Mass of detected protein : 28 kDa

Uniprot ID : Q04917

KGNC name : YWHAH

RRID # : AB_2572217

Purification : Affinity purified at 1 mg/mL

Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.

Validated applications : WB | IF/ICC | IHC

Suggested Dilutions:

WB: 1:2 000. IF/IHC: 1:500-1:1 000.

Datasheet

Link to 14-3-3η (14-3-3 eta) Antibody – MCA-3G12 on EnCor’s website

References :

1. Pachter, J and Liem, RKH. Alpha-Internexin, a 66-kD intermediate filament-binding protein from mammalian central nervous tissues. J Cell Biol 101:1316-22 (1985)

2. McGraw et al. Axonally transported peripheral signals regulate alpha-internexin expression in regenerating motoneurons. J Neurosci 22:4955-63 (2002)

3. Evans J. et al. Characterization of mitotic neurons derived from adult rat hypothalamus and brain stem. J. Neurophysiol. 87:1076-85 (2002).