Microtubule Associated Protein tau, MAPT Antibody - CPCA-Tau

Encor Chicken Polyclonal to Microtubule Associated Protein Tau, MAPT

Tau is a relatively low molecular weight member of the microtubule associated protein or MAP family. Most of these proteins were discovered since microtubules can be polymerized in cell homogenates and pelleted out by centrifugation, typically taking MAP proteins with them (1,2). This early work showed that tau protein facilitated the polymerization of microtubules, and was therefore given the name τ, the Greek letter tau, since it promoted tubule formation. The protein is now usually referred to simply as tau or by the HGNC name which is MAPT.
Tau is heavily concentrated in axons of neurons, but may also be found in dendrites and in some non neuronal cells. Much interest has focused on tau as it is a major component of the neurofibrillary tangles of Alzheimer’s disease (3,4). Tau in neurofibrillary tangles is typically heavily and aberrantly phosphorylated, and it is believed that phosphorylation may be involved in tangle formation. In addition, numerous different point mutations in the tau gene are causative of Fronto-temporal dementia with Parkinsonism linked to chromosome 17 (FTDP-17, see 5).

There is one mammalian tau gene which produces at least 9 different proteins by alternate transcription. In the central nervous system 6 isoforms predominant which either include or do not include three short exon coded inserts. These proteins range is size from 352-441 amino acids and run on SDS-PAGE gels as multiple bands ranging from 48-67 kDa. In peripheral nervous system a form called “big tau” predominates, another alternate transcript which includes a 254 amino acid insert (6). This form of tau is found in small amounts in the brain also, in cranial nerve motor nuclei and sensory processes of most sensory ganglia, and runs on SDS-PAGE with an apparent molecular weight of 100 kDa (7).

Each tau protein contains 3 or 4 copies of an 18 amino acid peptide which are responsible for binding to the microtubules and are similar to those found in MAP2 and other members of the MAP family. Tau is a highly charged acidic protein with few hydrophobic residues which belongs to the family of “intrinsically unstructured proteins“. As with GAP43, MARCKS and several other similar proteins, tau isoforms run on SDS-PAGE much more slowly than expected from their actual molecular weight.

Our antibody was made against a recombinant construct expressed in and purified from E. coli and which corresponded to the shortest version of the various tau proteins, so the antibody is expected to bind to all tau isoforms.

HGNC name(s) : MAPT

Host : Chicken

Clonality : Polyclonal

ID : EnCor Biotechnology Microtubule Associated Protein t, MAPT Tau

Reactivity : Human | Horse | Cow | Pig | Rat | Mouse

Isotype : IgY

Conjugation : none

Immunogen : Recombinant full length shortest human isoform

Mass of detected protein : 48-67 kDa

Uniprot ID : P10636

KGNC name : MAPT

RRID # : AB_2572348

Purification : Concentrated IgY preparation

Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.

Validated applications : WB | IF/ICC | IHC

Suggested Dilutions:

WB: 1:10 000. IF/ICC and IHC: 1:1 000.

Datasheet

Link to on EnCor’s website

References :

1. Weingarten, M. D., Lockwood, A. H., Hwo, S.-Y. and Kirschner, M. W. A protein factor essential for microtubule assembly. PNAS 72:1858–62 (1975).

2. Cleveland, D. W., Hwo, S. Y., Kirschner, M. W. Purification of tau, a microtubule-associated protein that induces assembly of microtubules from purified tubulin. J Mol. Biol. 116:207-25 (1977).

3. Goedert, M., Spillantini, M. G. A century of Alzheimer’s disease. Science 314:777–81 (2006).

4. Ballatore, C., Lee, V. M., Trojanowski, J. Q. Tau-mediated neurodegeneration in Alzheimer’s disease and related disorders. Nat. Rev. Neurosci. 8:663–72 (2007).

5. Wolfe, M. S. Tau mutations in neurodegenerative diseases. J. Biol. Chem. 284:6021-6025 (2009).

6. Goedert M, Spillantini MG, Crowther RA. Cloning of a big tau microtubule-associated protein characteristic of the peripheral nervous system. PNAS 89:1983-7 (1992).

7. Boyne LJ, Tessler A, Murray M, Fischer I. Distribution of Big tau in the central nervous system of the adult and developing rat. J. Comp. Neurol. 358:279-93 (1995).