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Fox1/A2BP1 Antibody – MCA-1G10

  • Clonality : Mouse Monoclonal
  • Applications : WB | IF/ICC | IHC
  • Reactivity : Human | Horse | Cow | Pig | Rat | Mouse

$338.00$1,810.00 CAD

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Encor Mouse Monoclonal to Anti-Fox1

Fox1 is one of a family of 3 mammalian Fox1 homologues. Fox1 was discovered in C. elegans as a gene involved in sex determination, and the name Fox is an acronym of “Feminizing locus on X” (1).
The C. elegans Fox1 protein and its 3 mammalian homologues are all ~46 kDa proteins and each includes a central highly conserved RRM type RNA recognition motif, which corresponds to a small ~70 amino acid structure consisting of 4 beta strands and two alpha-helices. This region is identical in all three mammalian Fox1 family proteins. An alternate name for Fox 1 is ataxin-2 binding protein 1 (A2BP1), since it was discovered in a yeast two hybrid screen using ataxin-2 as bait (2).

As with the other Fox proteins, it is assumed that Fox1/A2BP1 has a role in the RNA splicing in the nervous system (3). Fox3, a protein also known as NeuN, is a widely used marker of neuronal nuclei and proximal cytoplasm (4, 5). Like Fox3, Fox1 is expressed in neuronal nuclei, but with a different pattern of expression than Fox3. For example, in the cerebellum, Fox3 does not stain Purkinje neurons and Golgi neurons, but these two neuron types are stained by Fox1 antibody (6).

Our antibody was raised against the N-terminal 100 amino acids of human Fox1 as expressed in and purified from E. coli. We did not use full length Fox1 as the immunogen since the three mammalian Fox1 homologues, namely Fox1, Fox2 and Fox3, include virtually identical RRM motifs. The N-terminal region of the three molecules are much more variable in the three molecules so antibodies specific for each of the three molecules can therefore be generated.

The HGNC name for this protein is RBFOX1.


HGNC name(s) : RBFOX1


Host : Mouse


Clonality : Monoclonal


ID : EnCor Biotechnology Fox1/A2BP1 1G10


Reactivity : Human | Horse | Cow | Pig | Rat | Mouse


Isotype : IgG1


Conjugation : none


Immunogen : N-terminal 100 amino acids of human Fox1


Mass of detected protein : 46 and 48 kDa


Uniprot ID : Q9NWB1


KGNC name : RBFOX1


RRID # : AB_2572266


Purification : Affinity purified at 1 mg/mL


Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.


Validated applications : WB | IF/ICC | IHC


Suggested Dilutions:

WB: 1:1 000-1:2 000. ICC/IF and IHC: 1:500-1:1 000.


Datasheet


Link to on EnCor’s website


References :

1. Hodgkin J, Zellan JD, Albertson DG. Identification of a candidate primary sex determination locus, fox-1, on the X chromosome of Caenorhabditis elegans. Development 120:3681-3689 (1994).

2. Shibata H, Huynh DP, Pulst SM. A novel protein with RNA-binding motifs interacts with ataxin-2. Hum Mol Genet. 39:1303-13 (2000).

3. Underwood JG, Boutz PL, Dougherty JD, Stoilov P and Black DL. Homologues of the Caenorhabditis elegans Fox-1 protein are neuronal splicing regulators in mammals. Mol. Cell. Biol. 25:10005-10016 (2005).

4. Mullen RJ, Buck CR, Smith AM. NeuN, a neuronal specific nuclear protein in vertebrates. Development 116:201-211 (1994).

5. Kim KK, Adelstein RS, Kawamoto S. Identification of neuronal nuclei (NeuN) as Fox-3, a new member of the Fox-1 gene family of splicing factors. J. Biol. Chem. 284:31052-31061 (2009).

6. Kim KK, Kim YC, Adelstein RS, Kawamoto S. Fox-3 and PSF interact to activate neural cell-specific alternative splicing. Nucleic Acids Res. 39:3064-78 (2011).


Additional information

Format

50 ul, 100 ul, 500 ul

Supplier

Host

Mouse

Clonality

Monoclonal

Conjugation

None

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