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Neurofilament NF-H Antibody – MCA-NAP4

  • Clonality : Mouse Monoclonal
  • Applications : WB | IF/ICC | IHC
  • Reactivity : Human | Horse | Cow | Pig | Chicken | Rat | Mouse

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Encor Mouse Monoclonal to Neurofilament NF-H 

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDa, with some variability across species boundaries.
Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful in the diagnostics of neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (also known as Lou Gehrig’s disease) and Alzheimer’s disease.

MCA-AH1 is one of numerous antibodies which reacts preferentially with the axonal phosphorylated forms of NF-H. Interestingly these phosphorylated forms of NF-H are normally restricted to axons, while less phosphorylated forms are found in dendrites. However in numerous damage and disease states, phosphorylated NF-H can be detected with MCA-AH1 in dendritic and perikaryal neurofilaments. The HGNC name for this protein is NEFH.

MCA-NAP4 is a mouse monoclonal antibody raised against a preparation of native NF-H purified from pig spinal cord. MCA-NAP4 is an IgG1 class antibody with a k light chain. It recognizes phosphorylated NF-H KSP (lysine-serine-proline) type sequences. In some species there is some cross-reactivity with the related phosphorylated KSP sequences found in the related neurofilament subunit NF-M. The antibody recognizes NF-H strongly in all mammals tested to date and also in chicken. It recognizes neurofilaments in frozen sections in tissue culture and in formalin fixed sections.


HGNC name(s) : NEFH


Host : Mouse


Clonality : Monoclonal


ID : EnCor Biotechnology Neurofilament NF-H NAP4


Reactivity : Human | Horse | Cow | Pig | Chicken | Rat | Mouse


Isotype : IgG1


Conjugation : none


Immunogen : Purified bovine


Mass of detected protein : 200 kDa


Uniprot ID : P12036


KGNC name : NEFH


RRID # : AB_2572359


Purification : Ascites


Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.


Validated applications : WB | IF/ICC | IHC


Suggested Dilutions: 

WB: 1:10 000. ICC/IF and IHC: 1:1 000.


Datasheet


Link to on EnCor’s website 


References :

1. Harris, J., Ayyub, C. and Shaw G. A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62 1991.


Additional information

Format

50 ul, 100 ul, 500 ul

Supplier

Host

Mouse

Clonality

Monoclonal

Conjugation

None

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