Nestin Antibody – MCA-4D11

Encor Mouse monoclonal antibody to Nestin

Nestin is a member of the class IV intermediate filament protein family which is expressed in neuronal stem cells, which is the origin of the name nestin (1). Nestin was originally identified as a result of the production of a series of monoclonal antibodies directed against epitopes expressed on formalin fixed embryo day 15 rat spinal cord (2). One of these antibodies, called Rat 401, stained fibrous profiles in the developing nervous system, but not in the mature nervous system. By screening bacteriophage expression libraries with Rat 401, Lendahl et al. (1) were able to isolate a cDNA encoding the protein to which Rat 401 antibody bound.

The protein proved to be an unusual member of the intermediate filament family, containing an alpha-helical region homologous to that found in keratin and neurofilament subunits. The protein was named Nestin as, in the developing nervous system, it is found in neural stem cells. The Human Genome Organization (HUGO) Gene Nomenclature Committee (HGNC) name for Nestin is NES.

The Nestin protein has a very short N-terminal region in front of the alpha-helical region and a very long and repetitive C-terminal region. The molecular weight of human Nestin as determined by SDS-PAGE mobility is about 240 kDa. However the real molecular weight is considerably less than this, at 177 kDa, the disparity being likely due to the highly charged region of the C-terminal segment.

Nestin is relatively poorly conserved in protein sequence across species boundaries, so that the mouse and human proteins have an overall identity of only 62%. As a result antibodies to the human protein often fail to recognize the rodent homologue and vice versa. However MCA-4D11 stains both rodent and human Nestin. Antibodies to Nestin are widely used to identify neural stem cells.

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HGNC name(s) : NES

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Host : Mouse

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Clonality : Monoclonal

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ID : EnCor Biotechnology Nestin 4D11

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Reactivity : Human | Horse | Cow | Pig | Chicken | Rat | Mouse

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Isotype : IgG1

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Conjugation : none

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Immunogen : Amino acids 317-630 of human protein

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Mass of detected protein : ~240 kDa

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Uniprot ID : P48681

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KGNC name : NES

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RRID # : AB_2572355

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Purification : Ascites

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Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.

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Validated applications : WB | IF/ICC | IHC

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Suggested Dilutions:

WB: 1:5 000
IF/IHC: 1:500.

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Datasheet

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Link to on EnCor’s website

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References :

1. Lendahl U, Zimmerman LB and McKay RD. CNS stem cells express a new class of intermediate filament protein. Cell 60:585-95 (1990).

2. Hockfield S and McKay RD. Identification of major cell classes in the developing mammalian nervous system. J. Neurosci. 5:3310-3328 (1985).

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