Green-Red photoconvertible fluorescent protein EosFP Antibody – RPCA-EosFP

Encor Rabbit Polyclonal Antibody to EosFP

Fluorescent proteins have become widely used in a variety of experimental paradigms since the characterization of the first one, Green Fluorescent Protein (GFP) about 20 years ago. Some of these fluorescent proteins have the useful property of changing their emission spectrum from green to red following irradiation with blue or UV light, which allows simple but powerful pulse chase type experiments to be performed using more or less standard fluorescence microscopes. We have expressed in E. coli an purified a protein originally isolated from Lobophyllia hemprichii, a type of stony coral. The protein was described in Wiedenannn et al. (2004) and named EosFP, after Eos, the goddess of dawn in Greek mythology. Upon appropriate irradiation the protein changes emission from 516 nm to 581 nm, which happens to fit very conveniently to typical green (~498 nm) and red (~594 nm) filters on fluorescence microscopes. The emission shift is due to an irreversible covalent modification in the fluorochrome and is dependent on the His residue in the His-Tyr-Gly sequence that produces the fluorescence. The original coral protein was mutagenized to prevent tetramerization and dimerization, a requirement if the protein is to be used for fusion protein generation useful for FRET and similar techniques. A recent advance was the development of CaMPARI, an acronym for “Calcium-Modulated Photoactivatable Ratiometric Integrator” (2). This construct contains the GCaMP Calcium indicator fused to two EosFP domains, and will only transit from green to red when there is a coincidence between Calcium level elevation and the appropriate wavelength of light. This allows functional mapping of cellular activation in real time in appropriate transgenic animals (2)

HGNC name(s) : n/a

Host : Rabbit

Clonality : Polyclonal

ID : EnCor Biotechnology Green-Red photoconvertible fluorescent protein EosFP

Reactivity : n/a

Isotype : IgG

Conjugation : none

Immunogen : Recombinant full length

Mass of detected protein : ~25 kDa

Uniprot ID :

KGNC name : n/a

RRID # : AB_2572312

Purification : Serum

Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.

Validated applications : WB | IF/ICC | IHC

Suggested Dilutions:

WB: 1:1 000-5 000
IF/IHC: 1:5 000

Datasheet

Link to on EnCor’s website

References :

1. Wiedenmann J, Ivanchenko S, Oswald F, Schmitt F, Röcker C, Salih A, Spindler KD, Nienhaus GU. EosFP, a fluorescent marker protein with UV-inducible green-to-red fluorescence conversion. PNAS 101:15905-10 (2004).

2. Fosque BF, Sun Y, Dana H, Yang CT, Ohyama T, Tadross MR, Patel R, Zlatic M, Kim DS, Ahrens MB, Jayaraman V, Looger LL, Schreiter ER. Neural circuits. Labeling of active neural circuits in vivo with designed calcium integrators. Science 347:755-60 (2015).