TransNGS Tn5 DNA Library Prep Kit for Illumina® (50 ng DNA) – KP101

TransNGS Tn5 DNA Library Prep Kit for Illumina® (50 ng DNA)

TransNGS™ Tn5 DNA Library Prep Kit for Illumina® is designed to prepare genomic DNA libraries compatible with the Illumina® sequencers. In vitro adapter insertion and DNA fragmentation are performed in a single tube. Sequencing-ready libraries can be prepared in 2 hours with only 50 ng of starting genomic DNA.

The TransNGS™ Tn5 Library Prep Kit for Illumina® is compatible with other TransNGS series products. We recommend using the Tn5 Indexing Kit.

Features of the TransNGS Tn5 DNA Library Prep Kit for Illumina®

• Rapid and efficient : Sequencing-ready libraries in 2 hours.
• Low input DNA requirement : Only 50 ng of starting genomic DNA is required for high-diversity and high-quality NGS librairies.

Components included with the TransNGS Tn5 DNA Library Prep Kit for Illumina®

DNA Input Recommendations for Tn5 DNA Library Prep

The quality and quantity of the input genomic DNA is critical for DNA Library Prep. DNA should be purified and then dissolved in ddH2O or 10 mM Tris-HCl (pH8.0) with the OD260/OD280 between 1.8~2.0. Fluorescent dye measurement (i.e Qubit or PicoGreen) is highly recommended to determine DNA concentration. Avoid using spectrophotometry for DNA quantification (i.e UV absorbance or Nanodrop).

Principle of Tn5 DNA Library Prep

Tn5 Library Sequence

5’-AATGATACGGCGACCACCGAGATCTACAC-[i5]-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGXXXXXXXX-CTGTCTCTTATACACATCTCCGAGCCCACGAGAC-[i7]-ATCTCGTATGCCGTCTTCTGCTTG-3’
i5: Index 2, 8 bases;
i7: Index 1, 8 bases;
-XXXXXXXX-: inserted library DNA.

Typical DNA Yield

Representative Library outputs

Library without size selection – Human genomic library prepared with this kit, purified with 1.0 MagicPure DNA Size Selection Magnetic Beads;
Library without size selection – Human genomic library prepared with this kit, then size selected under 0.75+0.2; 0.63+0.15; or 0.54+0.12.

Note about using Tn5 transposase for DNA Library Preparation

All systems have bias resulting from many factors. The TransNGS™ Tn5 DNA Library Prep Kit for Illumina® has a slight bias from Tn5 Transposase insertion. The preference recognition sequences are listed below.

Manual & Datasheet

KP101 TransNGS Tn5 DNA Library Prep Kit for Illumina