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TransNGS Library Quantification Kit for Illumina – KQ101

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TransNGS Library Quantification Kit for Illumina

TransNGS™ Library Quantification Kit for Illumina® provides all reagents needed for qPCR-based quantification of DNA libraries prepared for Illumina next generation sequencing platforms. Five linear DNA Standards with different GC contents are provided for customers to choose from. The kit also contains PCR primers which target the P5 and P7 adaptor sequences and the TransNGS Library Quantification qPCR SuperMix (2x) for library amplifications.

The TransNGS™ Library Quantification Kit is formulated for the quantification of DNA libraries prepared for Illumina next-generation sequencing platforms using P5 and P7 adaptor sequences,


Features of NGS Library Quantification Kit for Illumina

  • DNA standards with different GC content : Five sets of linear DNA Standards (25%, 37.5%, 50%, 62.5%, and 75% of GC contents) to choose from.
  • Pre-dilution : Each DNA Standard contains six 10-fold dilution series of a linear 420 bp template.
  • qPCR-ready : TransNGS Library Quantification qPCR SuperMix (2x) enables accurate library amplification with various GC contents.

Contents of the TransNGS Library Quantification Kit for Illumina

KQ101 content - Trans NGS Library Quantification Kit for Illumina


Table1 DNA Standard Concentration Copy Number

KQ101 - Table1 DNA Standard Concentration Copy Number


Procedure for NGS Library Quantification using KQ101

KQ101 - Procedure for NGS Library Quantification

KQ101 - How to choose the DNA standards for NGS library quantification


Example of NGS Library Quantification

Two DNA libraries were prepared from 50 ng of Arabidopsis thaliana genomic DNA and 50 ng of Escherichia coli genomic DNA using TransNGS Tn5 DNA Library Prep Kit for Illumina®. The average fragment length and concentration of these 2 libraries were measured with Agilent Bioanalyzer (see table below).
An initial 1/10,000 dilution and one additional 2-fold (i.e. 1/20,000) dilution of these two libraries was prepared. Because the GC content of genomic DNA of Arabidopsis thaliana is about 36%, DNA Standard with 37.5% of GC contents was used to measure the library concentration. And the GC content of Escherichia coli is about 50%, DNA Standard with 50% of GC contents was used for Escherichia Coli library. The results and the calculated data are shown in the table below.

KQ101 - Example of NGS Library Quantification table A

KQ101 - Example of NGS Library Quantification table B

KQ101 - Example of NGS Library Quantification figure 2

KQ101 - Example of NGS Library Quantification figure 3


Manual & Datasheet

KQ101 TransNGS Library Quantification Kit for Illumina®

 

Additional information

Format

1 ml (100 rx), 5 x 1ml (500 rx)

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